概述

  • 产品名称Anti-NuMA抗体
    参阅全部 NuMA 一抗
  • 描述
    兔多克隆抗体to NuMA
  • 经测试应用适用于: ICC/IF, WB, IHC-Fr, IHC-P, IPmore details
  • 种属反应性
    与反应: Mouse, Chicken, Human
    预测可用于: all Mammals
  • 免疫原

    Recombinant full length protein corresponding to Human NuMA.

  • 阳性对照
    • HeLa whole cells.

性能

应用

Our Abpromise guarantee covers the use of ab36999 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/500.
WB 1/1000. Detects a band of approximately 220 kDa (predicted molecular weight: 238 kDa).
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IP 1/10 - 1/500.

靶标

  • 功能May be a structural component of the nucleus.
  • 细胞定位Nucleus. Chromosome. Dissociates from condensing chromosomes during early prophase, before the complete disintegration of the nuclear lamina. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Centrophilin stabilizes mitotic spindle in mitotic cells antibody
    • NMP 22 antibody
    • Nuclear matrix protein 22 antibody
    • Nuclear mitotic apparatus protein 1 antibody
    • Nuclear mitotic apparatus protein antibody
    • NUMA 1 antibody
    • NUMA antibody
    • NuMA protein antibody
    • NUMA1 antibody
    • NUMA1_HUMAN antibody
    • SP H antigen antibody
    • SP-H antigen antibody
    • Structural nuclear protein antibody
    see all

Anti-NuMA antibody 图像

  • ab36999 at 1/1000 dilution, staining human HeLa cells fixed with 3.5% formaldehyde, by immunofluorescence, showing the typical pattern for NuMa in the cell nucleus during interphase and on spindles during mitosis.

Anti-NuMA antibody (ab36999)参考文献

This product has been referenced in:
  • Platani M  et al. Mio depletion links mTOR regulation to Aurora A and Plk1 activation at mitotic centrosomes. J Cell Biol 210:45-62 (2015). ICC/IF ; Human . Read more (PubMed: 26124292) »
  • Kulukian A  et al. Epidermal development, growth control, and homeostasis in the face of centrosome amplification. Proc Natl Acad Sci U S A 112:E6311-20 (2015). IHC-Fr ; Mouse . Read more (PubMed: 26578791) »

See all 13 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Dog Cell (MDCK cells)
Specification MDCK cells
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1%
Fixative Paraformaldehyde
Username

Dr. Inma Banon

Verified customer

提交于 Sep 23 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"