概述

  • 产品名称
  • 描述
    兔多克隆抗体to NuMA
  • 经测试应用
    适用于: ICC/IF, WB, IHC-Fr, IHC-P, IPmore details
  • 种属反应性
    与反应: Mouse, Chicken, Human
    预测可用于: Mammal
  • 免疫原

    Recombinant full length protein corresponding to Human NuMA.

  • 阳性对照
    • HeLa whole cells.

性能

应用

Our Abpromise guarantee covers the use of ab36999 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/500.
WB 1/1000. Detects a band of approximately 220 kDa (predicted molecular weight: 238 kDa).
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IP 1/10 - 1/500.

靶标

  • 功能
    May be a structural component of the nucleus.
  • 细胞定位
    Nucleus. Chromosome. Dissociates from condensing chromosomes during early prophase, before the complete disintegration of the nuclear lamina. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Centrophilin stabilizes mitotic spindle in mitotic cells antibody
    • NMP 22 antibody
    • Nuclear matrix protein 22 antibody
    • Nuclear mitotic apparatus protein 1 antibody
    • Nuclear mitotic apparatus protein antibody
    • NUMA 1 antibody
    • NUMA antibody
    • NuMA protein antibody
    • NUMA1 antibody
    • NUMA1_HUMAN antibody
    • SP H antigen antibody
    • SP-H antigen antibody
    • Structural nuclear protein antibody
    see all

Anti-NuMA antibody 图像

  • ab36999 at 1/1000 dilution, staining human HeLa cells fixed with 3.5% formaldehyde, by immunofluorescence, showing the typical pattern for NuMa in the cell nucleus during interphase and on spindles during mitosis.

Anti-NuMA antibody (ab36999)参考文献

This product has been referenced in:
  • Pointer KB  et al. Association of collagen architecture with glioblastoma patient survival. J Neurosurg 126:1812-1821 (2017). Read more (PubMed: 27588592) »
  • Petridou NI & Skourides PA A ligand-independent integrin ß1 mechanosensory complex guides spindle orientation. Nat Commun 7:10899 (2016). Read more (PubMed: 26952307) »

See all 21 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Dog Cell (MDCK cells)
Specification
MDCK cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1%
Fixative
Paraformaldehyde
Username

Dr. Inma Banon

Verified customer

提交于 Sep 23 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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