The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 56 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
NUFIP1 contains 1 C2H2- type zinc finger and binds RNA. It interacts with FMR1. NUFIP1 may be associated with preinitiation complexes, open transcription complexes, and elongation complexes. It may facilitate ATP-dependent dissociation of hyperphosphorylated pol II from open transcription complexes.