概述

  • 产品名称Anti-Nuf2抗体
    参阅全部 Nuf2 一抗
  • 描述
    兔多克隆抗体to Nuf2
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Chicken, Human
    预测可用于: Rhesus monkey, Gorilla
  • 免疫原

    Synthetic peptide:

    CGGDSYAKIDEKTAELKRKMFKMS

    , corresponding to C terminal amino acids 443-463 of Human Nuf2.

  • 阳性对照
    • HeLa cells and cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.05% Sodium azide
    Constituent: 99% Whole serum
  • 纯度Whole antiserum
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab122962 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).
ICC/IF 1/200 - 1/1000. Methanol fixation is recommended for immunofluorescence staining.

靶标

  • 功能Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
  • 序列相似性Belongs to the NUF2 family.
  • 翻译后修饰Can be phosphorylated by AURKA and AURKB.
  • 细胞定位Nucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cancer/testis antigen 106 antibody
    • CDCA 1 antibody
    • CDCA1 antibody
    • Cell division cycle associated 1 antibody
    • Cell division cycle associated protein 1 antibody
    • Cell division cycle-associated protein 1 antibody
    • CT106 antibody
    • hNuf 2 antibody
    • hNuf2 antibody
    • hNuf2R antibody
    • hsNuf2 antibody
    • Kinetochore protein Nuf2 antibody
    • Nuf 2 antibody
    • NUF2 antibody
    • NUF2 NDC80 kinetochore complex component antibody
    • NUF2 NDC80 kinetochore complex component homolog antibody
    • NUF2, S. cerevisiae, homolog of antibody
    • NUF2_HUMAN antibody
    • NUF2R antibody
    see all

Anti-Nuf2 antibody 图像

  • Anti-Nuf2 antibody (ab122962) at 1/5000 dilution + HeLa cell lysate

    Predicted band size : 54 kDa
  • ab122962 at 1/500 dilution staining Nuf2 in Methanol-fixed HeLa cells by Immunofluorescence (green) showing localization to kinetochores at the stages of prometaphase to anaphase. Chromosomes were stained by DAPI (violet).

Anti-Nuf2 antibody (ab122962)参考文献

This product has been referenced in:
  • Hu P  et al. siRNA-mediated knockdown against NUF2 suppresses pancreatic cancer proliferation in vitro and in vivo. Biosci Rep 35:N/A (2015). WB, IHC-P ; Human . Read more (PubMed: 25370920) »
  • Xu P  et al. B56-PP2A regulates motor dynamics for mitotic chromosome alignment. J Cell Sci 127:4567-73 (2014). WB ; Human . Read more (PubMed: 25179604) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Colon carcinoma)
Permeabilization No
Specification Colon carcinoma
Fixative Methanol
Username

Dr. Eleni Petsalaki

Verified customer

提交于 Feb 01 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"