Anti-NUDT18抗体(ab82997)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to NUDT18
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 252-301 (KGLLGLQHLG RDHSDGICLN VLVTVAFRSP GIQDEPPKVR GENFSWWKVM) of Human NUDT18 (NP_079091).

  • 阳性对照
    • Transfected 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab82997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-NUDT18 antibody 图像

  • Anti-NUDT18 antibody (ab82997) at 1 µg/ml (in 5% skim milk / PBS buffer) + Transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 35 kDa
    Observed band size : 35 kDa

实验方案

Anti-NUDT18 antibody (ab82997)参考文献

ab82997 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Sample
Mouse Cell lysate - whole cell (HEK293T cells transfected)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
10 µg
Specification
HEK293T cells transfected
Blocking step
Blocking Ace as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
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提交于 Apr 04 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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