概述

  • 产品名称
    Anti-Nudel (phospho T245)抗体
    参阅全部 Nudel 一抗
  • 描述
    兔多克隆抗体to Nudel (phospho T245)
  • 经测试应用
    适用于: IHC-Fr, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic phosphopeptide corresponding to amino acid residues surrounding T245 of human Nudel conjugated to KLH

  • 阳性对照
    • Human breast carcinoma tissue.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 纯化说明
    ab60006 is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2 step procedure with the control and phosphorylated peptides. The phospho specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab60006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration. PubMed: 24785679
ELISA 1/1000.
IHC-P 1/50 - 1/100.

靶标

  • 功能
    Required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Also required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Plays a role, together with DISC1, in the regulation of neurite outgrowth. Required for mitosis in some cell types but appears to be dispensible for mitosis in cortical neuronal progenitors, which instead requires NDE1. Facilitates the polymerization of neurofilaments from the individual subunits NEFH and NEFL.
  • 组织特异性
    Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.
  • 序列相似性
    Belongs to the nudE family.
  • 发展阶段
    Expression peaks in mitosis.
  • 翻译后修饰
    Phosphorylated in mitosis. Can be phosphorylated by CDK1, CDK5 and MAPK1. Phosphorylation by CDK5 promotes interaction with KATNA1 and YWHAE.
  • 细胞定位
    Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Localizes to the cell body of the motor neurons and colocalizes with assembled neurofilaments within axonal processes. Localizes to the microtubules of the manchette in elongated spermatids. Colocalizes with DISC1 in the perinuclear region, including the centrosome (By similarity). Localizes to the interphase centrosome and the mitotic spindle. Localizes to the kinetochore in a CENPF-dependent manner.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A. nidulans antibody
    • DKFZp451M0318 antibody
    • ENDOOLIGOPEPTIDASE A antibody
    • EOPA antibody
    • MITAP 1 antibody
    • MITAP1 antibody
    • Mitosin associated protein 1 antibody
    • Mitosin associated protein MITAP1 antibody
    • Mitosin-associated protein 1 antibody
    • Ndel 1 antibody
    • NDEL1 antibody
    • NDEL1_HUMAN antibody
    • Nuclear distribution gene E like homolog 1 antibody
    • Nuclear distribution protein nudE like 1 antibody
    • Nuclear distribution protein nudE-like 1 antibody
    • NUDE like protein antibody
    • NudE nuclear distribution gene E homolog like 1 A. nidulans antibody
    • NudE nuclear distribution gene E homolog like 1 antibody
    • NUDEL antibody
    • Protein Nudel antibody
    see all

图片

  • ab60006 at 1/50 dilution staining Nudel in Human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. The primary antibody was peroxidase conjugated to the secondary antibody, followed by AEC staining.

文献

This product has been referenced in:
  • Pei Z  et al. The expression and roles of Nde1 and Ndel1 in the adult mammalian central nervous system. Neuroscience 271:119-36 (2014). WB, IHC-Fr ; Mouse . Read more (PubMed: 24785679) »

See 1 Publication for this product

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