RabMAb

Anti-Nudel抗体[EPR5068] (ab124895)

概述

  • 产品名称Anti-Nudel抗体[EPR5068]
    参阅全部 Nudel 一抗
  • 描述
    兔单克隆抗体[EPR5068] to Nudel
  • 经测试应用适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt,ICC/IF or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues near the N-terminus of Human Nudel. (Uniprot: Q9GZM8)

  • 阳性对照
    • Human Brain tissue, SH-SY5Y lysate, HeLa lysate, Jurkat lysate, 293T lysate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab124895 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
IHC-P 1/50 - 1/100. Antigen retrieval is recommended.
  • 应用说明Is unsuitable for Flow Cyt,ICC/IF or IP.
  • 靶标

    • 功能Required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Also required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Plays a role, together with DISC1, in the regulation of neurite outgrowth. Required for mitosis in some cell types but appears to be dispensible for mitosis in cortical neuronal progenitors, which instead requires NDE1. Facilitates the polymerization of neurofilaments from the individual subunits NEFH and NEFL.
    • 组织特异性Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.
    • 序列相似性Belongs to the nudE family.
    • 发展阶段Expression peaks in mitosis.
    • 翻译后修饰Phosphorylated in mitosis. Can be phosphorylated by CDK1, CDK5 and MAPK1. Phosphorylation by CDK5 promotes interaction with KATNA1 and YWHAE.
    • 细胞定位Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Localizes to the cell body of the motor neurons and colocalizes with assembled neurofilaments within axonal processes. Localizes to the microtubules of the manchette in elongated spermatids. Colocalizes with DISC1 in the perinuclear region, including the centrosome (By similarity). Localizes to the interphase centrosome and the mitotic spindle. Localizes to the kinetochore in a CENPF-dependent manner.
    • Information by UniProt
    • 数据库链接
    • 别名
      • A. nidulans antibody
      • DKFZp451M0318 antibody
      • ENDOOLIGOPEPTIDASE A antibody
      • EOPA antibody
      • MITAP 1 antibody
      • MITAP1 antibody
      • Mitosin associated protein 1 antibody
      • Mitosin associated protein MITAP1 antibody
      • Mitosin-associated protein 1 antibody
      • Ndel 1 antibody
      • NDEL1 antibody
      • NDEL1_HUMAN antibody
      • Nuclear distribution gene E like homolog 1 antibody
      • Nuclear distribution protein nudE like 1 antibody
      • Nuclear distribution protein nudE-like 1 antibody
      • NUDE like protein antibody
      • NudE nuclear distribution gene E homolog like 1 A. nidulans antibody
      • NudE nuclear distribution gene E homolog like 1 antibody
      • NUDEL antibody
      • Protein Nudel antibody
      see all

    Anti-Nudel antibody [EPR5068] 图像

    • All lanes : Anti-Nudel antibody [EPR5068] (ab124895) at 1/1000 dilution

      Lane 1 : SH-SY5Y lysate
      Lane 2 : HeLa lysate
      Lane 3 : Jurkat lysate
      Lane 4 : 293T lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 38 kDa
    • ab124895, at 1/50 dilution, staining Nudel in Formalin-fixed, Paraffin-embedded Human Brain tissue by Immunohistochemistry
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-Nudel antibody [EPR5068] (ab124895)参考文献

    ab124895 has not yet been referenced specifically in any publications.

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