概述

  • 产品名称Anti-Nudel抗体
    参阅全部 Nudel 一抗
  • 描述
    山羊多克隆抗体to Nudel
  • 特异性ab81975 is expected to recognize both reported isoforms (NP_001020750.1 and NP_110435.1)
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Chicken, Xenopus laevis, Zebrafish, Cynomolgus Monkey, Orangutan
  • 免疫原

    Synthetic peptide:

    CRNFAKDQASRKSY

    , corresponding to internal sequence amino acids 273 - 286 of Human Nudel (NP_001020750.1, NP_110435.1)

  • 阳性对照
    • Human Cerebellum lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab81975 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab81975 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 38 kDa.

靶标

  • 功能Required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Also required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Plays a role, together with DISC1, in the regulation of neurite outgrowth. Required for mitosis in some cell types but appears to be dispensible for mitosis in cortical neuronal progenitors, which instead requires NDE1. Facilitates the polymerization of neurofilaments from the individual subunits NEFH and NEFL.
  • 组织特异性Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.
  • 序列相似性Belongs to the nudE family.
  • 发展阶段Expression peaks in mitosis.
  • 翻译后修饰Phosphorylated in mitosis. Can be phosphorylated by CDK1, CDK5 and MAPK1. Phosphorylation by CDK5 promotes interaction with KATNA1 and YWHAE.
  • 细胞定位Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Localizes to the cell body of the motor neurons and colocalizes with assembled neurofilaments within axonal processes. Localizes to the microtubules of the manchette in elongated spermatids. Colocalizes with DISC1 in the perinuclear region, including the centrosome (By similarity). Localizes to the interphase centrosome and the mitotic spindle. Localizes to the kinetochore in a CENPF-dependent manner.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A. nidulans antibody
    • DKFZp451M0318 antibody
    • ENDOOLIGOPEPTIDASE A antibody
    • EOPA antibody
    • MITAP 1 antibody
    • MITAP1 antibody
    • Mitosin associated protein 1 antibody
    • Mitosin associated protein MITAP1 antibody
    • Mitosin-associated protein 1 antibody
    • Ndel 1 antibody
    • NDEL1 antibody
    • NDEL1_HUMAN antibody
    • Nuclear distribution gene E like homolog 1 antibody
    • Nuclear distribution protein nudE like 1 antibody
    • Nuclear distribution protein nudE-like 1 antibody
    • NUDE like protein antibody
    • NudE nuclear distribution gene E homolog like 1 A. nidulans antibody
    • NudE nuclear distribution gene E homolog like 1 antibody
    • NUDEL antibody
    • Protein Nudel antibody
    see all

Anti-Nudel antibody 图像

  • Anti-Nudel antibody (ab81975) at 0.1 µg/ml + Human Cerebellum lysate (in RIPA buffer) at 35 µg

    Predicted band size : 38 kDa
    Observed band size : 38 kDa
    Additional bands at : 39 kDa (possible isoform).

Anti-Nudel antibody (ab81975)参考文献

ab81975 has not yet been referenced specifically in any publications.

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