概述

  • 产品名称Anti-Nudel抗体
    参阅全部 Nudel 一抗
  • 描述
    兔多克隆抗体to Nudel
  • 特异性ab25959 recognises Nudel.
  • 经测试应用适用于: IP, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide (Human) - corresponding to 15 amino acids near the amino terminus of human Nudel.

  • 阳性对照
    • Jurkat cell lysate.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab25959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent dilution. PubMed: 17984054
ICC/IF Use a concentration of 2 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 38 kDa.Can be blocked with Human Nudel peptide (ab39840).

靶标

  • 功能Required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Also required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Plays a role, together with DISC1, in the regulation of neurite outgrowth. Required for mitosis in some cell types but appears to be dispensible for mitosis in cortical neuronal progenitors, which instead requires NDE1. Facilitates the polymerization of neurofilaments from the individual subunits NEFH and NEFL.
  • 组织特异性Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.
  • 序列相似性Belongs to the nudE family.
  • 发展阶段Expression peaks in mitosis.
  • 翻译后修饰Phosphorylated in mitosis. Can be phosphorylated by CDK1, CDK5 and MAPK1. Phosphorylation by CDK5 promotes interaction with KATNA1 and YWHAE.
  • 细胞定位Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Localizes to the cell body of the motor neurons and colocalizes with assembled neurofilaments within axonal processes. Localizes to the microtubules of the manchette in elongated spermatids. Colocalizes with DISC1 in the perinuclear region, including the centrosome (By similarity). Localizes to the interphase centrosome and the mitotic spindle. Localizes to the kinetochore in a CENPF-dependent manner.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A. nidulans antibody
    • DKFZp451M0318 antibody
    • ENDOOLIGOPEPTIDASE A antibody
    • EOPA antibody
    • MITAP 1 antibody
    • MITAP1 antibody
    • Mitosin associated protein 1 antibody
    • Mitosin associated protein MITAP1 antibody
    • Mitosin-associated protein 1 antibody
    • Ndel 1 antibody
    • NDEL1 antibody
    • NDEL1_HUMAN antibody
    • Nuclear distribution gene E like homolog 1 antibody
    • Nuclear distribution protein nudE like 1 antibody
    • Nuclear distribution protein nudE-like 1 antibody
    • NUDE like protein antibody
    • NudE nuclear distribution gene E homolog like 1 A. nidulans antibody
    • NudE nuclear distribution gene E homolog like 1 antibody
    • NUDEL antibody
    • Protein Nudel antibody
    see all

Anti-Nudel antibody 图像

  • Lane 1 : Anti-Nudel antibody (ab25959) at 0.5 µg/ml
    Lane 2 : Anti-Nudel antibody (ab25959) at 1 µg/ml
    Lane 3 : Anti-Nudel antibody (ab25959) at 2 µg/ml

    Lane 1 : Jurkat whole cell lysate.
    Lane 2 : Jurkat whole cell lysate.
    Lane 3 : Jurkat whole cell lysate.


    Predicted band size : 38 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
  • ab25959 at 2µg/ml staining Nudel in Jurkat cells by ICC/IF

  • Immunofluorescence of Nudel in Jurkat cells using ab25959 at 10 ug/ml.

Anti-Nudel antibody (ab25959)参考文献

This product has been referenced in:
  • Turgay Y  et al. SUN proteins facilitate the removal of membranes from chromatin during nuclear envelope breakdown. J Cell Biol 204:1099-109 (2014). Human . Read more (PubMed: 24662567) »
  • Leliveld SR  et al. Insolubility of disrupted-in-schizophrenia 1 disrupts oligomer-dependent interactions with nuclear distribution element 1 and is associated with sporadic mental disease. J Neurosci 28:3839-45 (2008). Read more (PubMed: 18400883) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (U2OS and Hela)
Specification U2OS and Hela
Fixative Methanol
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
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Dr. GUOJIE MAO

Verified customer

提交于 Dec 01 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"