antigen: PFEERSGVVP CGTPWGQWYQ TLEEVFIEVQ VPPGTRAQDI QCGLQSRHVA LSV, corresponding to amino acids 4-56 of Human NUDCD2 (Q8WVJ2).
Human kidney tissue. RT-4 and U-251 MG cell lysates. Human liver and tonsil tissue lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/250 - 1/500.
Contains 1 CS domain.
Information by UniProt
NudC domain containing 2 antibody
NudC domain-containing protein 2 antibody
NudC like protein 2 antibody
Immunocytochemistry/ Immunofluorescence - Anti-NUDCD2 antibody (ab122384)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm and cytoskeleton (microtubules). Recommended concentration of ab122384 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUDCD2 antibody (ab122384)
ab122384 at 1/500 dilution staining NUDCD2 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Western blot - Anti-NUDCD2 antibody (ab122384)
All lanes : Anti-NUDCD2 antibody (ab122384) at 1/250 dilution Lane 1 : RT-4 cell lysate Lane 2 : U-251 MG cell lysate Lane 3 : Human plasma lysate Lane 4 : Human liver tissue lysate Lane 5 : Human tonsil tissue lysate Developed using the ECL technique.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"