The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 62 kDa.
Use a concentration of 10 µg/ml.
功能May be required to maintain the proliferative capacity of stem cells and may play an important role in tumorigenesis.
组织特异性Increased levels in lung tissue in cancer patients.
序列相似性Belongs to the MMR1/HSR1 GTP-binding protein family. Contains 1 G (guanine nucleotide-binding) domain.
结构域The basic domain (B) allows nucleolar localization in the absence of GTP. The intermediate domain (I) inhibits nucleolar localization by the B domain and is required for exit from the nucleolus. Exit from the nucleolus to the nucleoplasm requires both the I and the acidic (A) domains, and may be triggered by GTP hydrolysis. In contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern.
细胞定位Nucleus. Nucleus > nucleolus. Shuttles between the nucleus and nucleolus.