Anti-Nucleostemin抗体(ab88882)

概述

  • 产品名称Anti-Nucleostemin抗体
    参阅全部 Nucleostemin 一抗
  • 描述
    小鼠多克隆抗体to Nucleostemin
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length Human Nucleostemin (AAH01024.1)

  • 阳性对照
    • Hela and LNCaP cell lysates, Nucleostemin transfected 293T cell lysate and Hela cells.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab88882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 62 kDa.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能May be required to maintain the proliferative capacity of stem cells and may play an important role in tumorigenesis.
  • 组织特异性Increased levels in lung tissue in cancer patients.
  • 序列相似性Belongs to the MMR1/HSR1 GTP-binding protein family.
    Contains 1 G (guanine nucleotide-binding) domain.
  • 结构域The basic domain (B) allows nucleolar localization in the absence of GTP. The intermediate domain (I) inhibits nucleolar localization by the B domain and is required for exit from the nucleolus. Exit from the nucleolus to the nucleoplasm requires both the I and the acidic (A) domains, and may be triggered by GTP hydrolysis.
    In contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern.
  • 细胞定位Nucleus. Nucleus > nucleolus. Shuttles between the nucleus and nucleolus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C77032 antibody
    • E2 induced gene 3 protein antibody
    • E2-induced gene 3 protein antibody
    • E2IG3 antibody
    • Estradiol induced nucleotide binding protein antibody
    • GNL3 antibody
    • GNL3_HUMAN antibody
    • Guanine nucleotide binding protein like 3 (nucleolar) antibody
    • Guanine nucleotide binding protein like 3 antibody
    • Guanine nucleotide-binding protein-like 3 antibody
    • MGC800 antibody
    • NNP47 antibody
    • Novel nucleolar protein 47 antibody
    • NS antibody
    • Nucleolar GTP binding protein 3 antibody
    • Nucleolar GTP-binding protein 3 antibody
    • Nucleostemin antibody
    • Putative nucleotide binding protein estradiol induced antibody
    see all

Anti-Nucleostemin antibody 图像

  • Anti-Nucleostemin antibody (ab88882) at 1 µg/ml + HeLa cell lysate at 50 µg

    Predicted band size : 62 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • Anti-Nucleostemin antibody (ab88882) at 1 µg/ml + LNCaP cell lysate at 50 µg

    Predicted band size : 62 kDa
    Observed band size : 62 kDa
  • All lanes : Anti-Nucleostemin antibody (ab88882) at 1 µg/ml

    Lane 1 : Nucleostemin transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 62 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • ab88882 at 10 ug/ml staining Nucleostemin in Hela cells by Immunofluorescence

Anti-Nucleostemin antibody (ab88882)参考文献

ab88882 has not yet been referenced specifically in any publications.

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