• 产品名称Anti-Nucleostemin抗体
    参阅全部 Nucleostemin 一抗
  • 描述
    兔多克隆抗体to Nucleostemin
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Horse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
  • 免疫原

    Synthetic peptide corresponding to a region between residues 1 and 50 of human Nucleostemin (NP_055181.3)

  • 阳性对照
    • HeLa, 293T and mouse 3T3 whole cell lysates.



Our Abpromise guarantee covers the use of ab70345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
IP Use at 1-4 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • 功能May be required to maintain the proliferative capacity of stem cells and may play an important role in tumorigenesis.
  • 组织特异性Increased levels in lung tissue in cancer patients.
  • 序列相似性Belongs to the MMR1/HSR1 GTP-binding protein family.
    Contains 1 G (guanine nucleotide-binding) domain.
  • 结构域The basic domain (B) allows nucleolar localization in the absence of GTP. The intermediate domain (I) inhibits nucleolar localization by the B domain and is required for exit from the nucleolus. Exit from the nucleolus to the nucleoplasm requires both the I and the acidic (A) domains, and may be triggered by GTP hydrolysis.
    In contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern.
  • 细胞定位Nucleus. Nucleus > nucleolus. Shuttles between the nucleus and nucleolus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C77032 antibody
    • E2 induced gene 3 protein antibody
    • E2-induced gene 3 protein antibody
    • E2IG3 antibody
    • Estradiol induced nucleotide binding protein antibody
    • GNL3 antibody
    • GNL3_HUMAN antibody
    • Guanine nucleotide binding protein like 3 (nucleolar) antibody
    • Guanine nucleotide binding protein like 3 antibody
    • Guanine nucleotide-binding protein-like 3 antibody
    • MGC800 antibody
    • NNP47 antibody
    • Novel nucleolar protein 47 antibody
    • NS antibody
    • Nucleolar GTP binding protein 3 antibody
    • Nucleolar GTP-binding protein 3 antibody
    • Nucleostemin antibody
    • Putative nucleotide binding protein estradiol induced antibody
    see all

Anti-Nucleostemin antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin carcinoma tissue labelling Nucleostemin with ab70345 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-Nucleostemin antibody (ab70345) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : mouse 3T3 whole cell lysate at 50 µg/ml

    Predicted band size : 62 kDa
    Observed band size : 62 kDa

    Exposure time : 10 seconds
  • Detection of Human Nucleostemin by Western Blot of Immunprecipitate. All lanes: ab70345 at 1µg/ml staining Nucleostemin in HeLa whole cell lysates immunoprecipitated at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane) using the following antibodies: Lane 1: an irrelevant antibody Lane 2: ab70345 Detection: Chemiluminescence with exposure times of 10 seconds

Anti-Nucleostemin antibody (ab70345)参考文献

ab70345 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab70345.
Please use the links above to contact us or submit feedback about this product.