The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/250 - 1/4000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Use at an assay dependent concentration. Detects a band of approximately 68 kDa (predicted molecular weight: 60 kDa).
Use a concentration of 1 µg/ml.
功能May be required to maintain the proliferative capacity of stem cells and may play an important role in tumorigenesis.
组织特异性Increased levels in lung tissue in cancer patients.
序列相似性Belongs to the MMR1/HSR1 GTP-binding protein family. Contains 1 G (guanine nucleotide-binding) domain.
结构域The basic domain (B) allows nucleolar localization in the absence of GTP. The intermediate domain (I) inhibits nucleolar localization by the B domain and is required for exit from the nucleolus. Exit from the nucleolus to the nucleoplasm requires both the I and the acidic (A) domains, and may be triggered by GTP hydrolysis. In contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern.
细胞定位Nucleus. Nucleus > nucleolus. Shuttles between the nucleus and nucleolus.
ICC/IF image of ab32682 stained mouse embryonic stem cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32682, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-Nucleostemin antibody (ab32682)参考文献
has not yet been referenced specifically in any publications.