Anti-Nucleostemin抗体(ab14293)

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概述

  • 产品名称
    Anti-Nucleostemin抗体
    参阅全部 Nucleostemin 一抗
  • 描述
    鸡多克隆抗体to Nucleostemin
  • 宿主
    Chicken
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Nucleostemin aa 1-280.
    Sequence:

    MKRPKLKKASKRMTCHKRYKIQKKVREHHRKLRKEAKKQGHKKPRKDPGV PNSAPFKEAL LREAELRKQRLEELKQQQKLDRQKELEKKRKLETNPDI KPSNVEPMEKEFGLCKTENKAK SGKQNSKKLYCQELKKVIEASDVVLE VLDARDPLGCRCPQVEEAIVQSGQKKLVLILNKS DLVPKENLESWLNY LKKELPTVVFRASTKPKDKGKITKRVKAKKNAAPFRSEVCFGKEGL WK LLGGFQETCSKAIRVGVIGFPNVGKSSIINSLKQEQMC

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明
    During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.

性能

应用

Our Abpromise guarantee covers the use of ab14293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/2000. Predicted molecular weight: 66 kDa.

靶标

  • 功能
    May be required to maintain the proliferative capacity of stem cells and may play an important role in tumorigenesis.
  • 组织特异性
    Increased levels in lung tissue in cancer patients.
  • 序列相似性
    Belongs to the MMR1/HSR1 GTP-binding protein family.
    Contains 1 G (guanine nucleotide-binding) domain.
  • 结构域
    The basic domain (B) allows nucleolar localization in the absence of GTP. The intermediate domain (I) inhibits nucleolar localization by the B domain and is required for exit from the nucleolus. Exit from the nucleolus to the nucleoplasm requires both the I and the acidic (A) domains, and may be triggered by GTP hydrolysis.
    In contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern.
  • 细胞定位
    Nucleus. Nucleus > nucleolus. Shuttles between the nucleus and nucleolus.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • C77032 antibody
    • E2 induced gene 3 protein antibody
    • E2-induced gene 3 protein antibody
    • E2IG3 antibody
    • Estradiol induced nucleotide binding protein antibody
    • GNL3 antibody
    • GNL3_HUMAN antibody
    • Guanine nucleotide binding protein like 3 (nucleolar) antibody
    • Guanine nucleotide binding protein like 3 antibody
    • Guanine nucleotide-binding protein-like 3 antibody
    • MGC800 antibody
    • NNP47 antibody
    • Novel nucleolar protein 47 antibody
    • NS antibody
    • Nucleolar GTP binding protein 3 antibody
    • Nucleolar GTP-binding protein 3 antibody
    • Nucleostemin antibody
    • Putative nucleotide binding protein estradiol induced antibody
    see all

图片

  • Western blot - Anti-Nucleostemin antibody (ab14293)
    Western blot - Anti-Nucleostemin antibody (ab14293)

    Western Blot Test: E coli-derived fusion protein as test antigen. Affi-anti- RGS6 IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. Arrow points to E2IG3 fusion protein.



    E coli-derived fusion protein as test antigen. Affi-anti- RGS6 IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. Arrow points to E2IG3 fusion protein.

实验方案

文献

ab14293 has not yet been referenced specifically in any publications.

发表研究结果有使用 ab14293？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Nucleostemin antibody

 Inconclusive
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Adult rat brain)
Specification
Adult rat brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
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Mr. Carl Hobbs

Verified customer

提交于 May 14 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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