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This product was produced with the following immunogens:
Synthetic peptide corresponding to Human Nucleophosmin aa 23-38.
Synthetic peptide corresponding to Human Nucleophosmin aa 226-240.
Our Abpromise guarantee covers the use of ab15440 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||1/100. Used at a dilution of 1/100 (see Abreview for further information).|
|WB||1/500 - 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 33 kDa).|
Western blot analysis of various cell lines (25ug/lane) labeling Neucleophosmin with ab15440 at 1/500. A HRP-conjugated goat anti-rabbit IgG (1/20000) was used as the secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"