Anti-Nucleophosmin抗体(ab114853)

概述

  • 产品名称Anti-Nucleophosmin抗体
    参阅全部 Nucleophosmin 一抗
  • 描述
    兔多克隆抗体to Nucleophosmin
  • 经测试应用适用于: IHC-P, IPmore details
    不适用于: WB
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 250-294 of Human Nucleophosmin (NP_002511.1).

  • 阳性对照
    • HeLa whole cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab114853 was affinity purified using an epitope specific to Nucleophosmin immobilized on a solid support.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab114853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 5-10 µg/mg of lysate.
  • 应用说明Is unsuitable for WB.
  • 靶标

    • 功能Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed:22528486).
    • 疾病相关A chromosomal aberration involving NPM1 is found in a form of non-Hodgkin lymphoma. Translocation t(2;5)(p23;q35) with ALK. The resulting chimeric NPM1-ALK protein homodimerize and the kinase becomes constitutively activated.
      A chromosomal aberration involving NPM1 is found in a form of acute promyelocytic leukemia. Translocation t(5;17)(q32;q11) with RARA.
      A chromosomal aberration involving NPM1 is a cause of myelodysplastic syndrome (MDS). Translocation t(3;5)(q25.1;q34) with MLF1.
      Defects in NPM1 are associated with acute myelogenous leukemia (AML). Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location.
    • 序列相似性Belongs to the nucleoplasmin family.
    • 翻译后修饰Acetylated at C-terminal lysine residues, thereby increasing affinity to histones.
      ADP-ribosylated.
      Phosphorylated at Ser-4 by PLK1 and PLK2. Phosphorylation at Ser-4 by PLK2 in S phase is required for centriole duplication and is sufficient to trigger centriole replication. Phosphorylation at Ser-4 by PLK1 takes place during mitosis. Phosphorylated by CDK2 at Ser-125 and Thr-199. Phosphorylation at Thr-199 may trigger initiation of centrosome duplication. Phosphorylated by CDK1 at Thr-199, Thr-219, Thr-234 and Thr-237 during cell mitosis. When these four sites are phosphorated, RNA-binding activity seem to be abolished. May be phosphorylated at Ser-70 by NEK2. The Thr-199 phosphorylated form has higher affinity for ROCK2. CDK6 triggers Thr-199 phosphorylation when complexed to Kaposi's sarcoma herpesvirus (KSHV) V-cyclin, leading to viral reactivation by reducing viral LANA levels.
      Sumoylated by ARF.
    • 细胞定位Nucleus, nucleolus. Nucleus, nucleoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Generally nucleolar, but is translocated to the nucleoplasm in case of serum starvation or treatment with anticancer drugs. Has been found in the cytoplasm in patients with primary acute myelogenous leukemia (AML), but not with secondary AML. Can shuttle between cytoplasm and nucleus. Co- localizes with the methylated form of RPS10 in the granular component (GC) region of the nucleolus. Colocalized with nucleolin and APEX1 in nucleoli. Isoform 1 of NEK2 is required for its localization to the centrosome during mitosis.
    • Information by UniProt
    • 数据库链接
    • 别名
      • B23 antibody
      • MGC104254 antibody
      • NO38 antibody
      • NPM antibody
      • NPM_HUMAN antibody
      • NPM1 antibody
      • Nucleolar phosphoprotein B23 antibody
      • Nucleolar protein NO38 antibody
      • Nucleophosmin (nucleolar phosphoprotein B23 numatrin) antibody
      • Nucleophosmin antibody
      • nucleophosmin nucleoplasmin family member 1 antibody
      • Nucleophosmin/nucleoplasmin family member 1 antibody
      • Numatrin antibody
      • OTTHUMP00000161024 antibody
      • OTTHUMP00000161025 antibody
      • OTTHUMP00000223397 antibody
      • OTTHUMP00000223398 antibody
      see all

    Anti-Nucleophosmin antibody 图像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling Nucleophosmin with ab114853 at 1/1000 (1µg/ml). Detection: DAB.
    • ab114853, precipitating Nucleophosmin at 10 µg/mg lysate; detection by WB utilised an alternative anti-Nucleophosmin antibody, raised against an upstream epitope (at 0.1 µg/ml).
      Lane 1; IP using an antibody to an upstream epitope. This antibody was also used for WB detection.
      Lane 2; IP using another antibody to an upstream epitope.
      Lane 3; IP using ab114853 at 10 µg/mg HeLa whole cell lysate.
      Lane 4; IP using control IgG.
      1 mg of lysate was used for IP, and 20% of IP was loaded. Detection utilised ECL with a 3 second exposure.

    Anti-Nucleophosmin antibody (ab114853)参考文献

    ab114853 has not yet been referenced specifically in any publications.

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