概述

  • 产品名称
    Anti-Nucleolin抗体
    参阅全部 Nucleolin 一抗
  • 描述
    兔多克隆抗体to Nucleolin
  • 经测试应用
    适用于: WB, IP, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cynomolgus monkey, Orangutan
  • 免疫原

    Synthetic peptide:

    VKLAKAGKNQGDPKKMA

    conjugated to KLH, corresponding to N terminal amino acids 2-17 of Human Nucleolin with a C-terminal added cysteine.

  • 阳性对照
    • Whole extract of mouse NIH3T3 cells or rat PC12 cells, or human HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab50279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 77 kDa.
IP Use a concentration of 1 - 2 µg/ml.

1-2 µg/ml immunoprecipitates Nucleolin from 250 ug RIPA lysate of human HeLa cells.

ICC/IF Use a concentration of 10 - 20 µg/ml.
Flow Cyt Use at an assay dependent concentration. PubMed: 20814900

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

图片

  • Indirect Immunofluorescence

    Human HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 10 µg/mL Anti-Nucleolin (ab50279). The antibody was developed with Goat Anti-Rabbit IgG, FITC conjugate.

文献

This product has been referenced in:
  • García-Rubio ML  et al. The Fanconi Anemia Pathway Protects Genome Integrity from R-loops. PLoS Genet 11:e1005674 (2015). ICC/IF . Read more (PubMed: 26584049) »
  • Bender BJ  et al. Dynamic and nucleolin-dependent localization of human cytomegalovirus UL84 to the periphery of viral replication compartments and nucleoli. J Virol 88:11738-47 (2014). Read more (PubMed: 25078694) »

See all 10 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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