概述

  • 产品名称Anti-Nucleolin抗体
    参阅全部 Nucleolin 一抗
  • 描述
    兔多克隆抗体to Nucleolin
  • 经测试应用适用于: WB, IP, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cynomolgus Monkey, Orangutan
  • 免疫原

    Synthetic peptide:

    VKLAKAGKNQGDPKKMA

    conjugated to KLH, corresponding to N terminal amino acids 2-17 of Human Nucleolin with a C-terminal added cysteine.

  • 阳性对照
    • Whole extract of mouse NIH3T3 cells or rat PC12 cells, or human HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab50279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 77 kDa.
IP Use a concentration of 1 - 2 µg/ml.

1-2 µg/ml immunoprecipitates Nucleolin from 250 ug RIPA lysate of human HeLa cells.

ICC/IF Use a concentration of 10 - 20 µg/ml.
Flow Cyt Use at an assay dependent concentration. PubMed: 20814900

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
  • 序列相似性Contains 4 RRM (RNA recognition motif) domains.
  • 翻译后修饰Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
  • 细胞定位Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C23 antibody
    • FLJ45706 antibody
    • MS1116 antibody
    • NCL antibody
    • Nucl antibody
    • NUCL_HUMAN antibody
    • Nucleolin antibody
    • Protein C23 antibody
    see all

Anti-Nucleolin antibody 图像

  • Indirect Immunofluorescence

    Human HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 10 µg/mL Anti-Nucleolin (ab50279). The antibody was developed with Goat Anti-Rabbit IgG, FITC conjugate.

Anti-Nucleolin antibody (ab50279)参考文献

This product has been referenced in:
  • Bender BJ  et al. Dynamic and nucleolin-dependent localization of human cytomegalovirus UL84 to the periphery of viral replication compartments and nucleoli. J Virol 88:11738-47 (2014). Read more (PubMed: 25078694) »
  • Peddigari S  et al. hnRNPL and nucleolin bind LINE-1 RNA and function as host factors to modulate retrotransposition. Nucleic Acids Res 41:575-85 (2013). WB ; Human . Read more (PubMed: 23161687) »

See all 9 Publications for this product

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