Anti-Nucleobindin 1抗体(ab26093)

概述

性能

应用

Our Abpromise guarantee covers the use of ab26093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1.5625e+006.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 54 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    Major calcium-binding protein of the Golgi. May have a role in calcium homeostasis.
  • 组织特异性
    Expressed both in fetal and adult heart, lung, liver, kidney and brain, and in adult skeletal muscle, placenta and pancreas.
  • 序列相似性
    Belongs to the nucleobindin family.
    Contains 2 EF-hand domains.
  • 结构域
    The EF-hand domains are unfolded in the absence of Ca(2+) and fold upon Ca(2+) addition.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Golgi apparatus > cis-Golgi network membrane. Cytoplasm. A small fraction of the protein may be cytoplasmic.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CALNUC antibody
    • FLJ40471 antibody
    • NUC antibody
    • NUCB 1 antibody
    • NUCB1 antibody
    • NUCB1_HUMAN antibody
    • Nucleobindin 1 antibody
    • Nucleobindin-1 antibody
    see all

Anti-Nucleobindin 1 antibody 图像

  • Anti-Nucleobindin 1 antibody (ab26093) at 1 µg/ml + HepG2 cell lysate 10ug.

    Predicted band size : 54 kDa

实验方案

Anti-Nucleobindin 1 antibody (ab26093)参考文献

ab26093 has not yet been referenced specifically in any publications.

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According to Swiss Prot: Q02818 (http://www.uniprot.org/uniprot/Q02818) the predicted size of this product should be 54kDa before any post translational modifications. This protein contains many phospho sites a...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (MDA-MB-231 breast cancer cell line)
Loading amount
40 µg
Specification
MDA-MB-231 breast cancer cell line
Gel Running Conditions
Reduced Denaturing (gel 10 %)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Oct 10 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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