Anti-Nucleobindin 1抗体(ab23360)

概述

  • 产品名称Anti-Nucleobindin 1抗体
    参阅全部 Nucleobindin 1 一抗
  • 描述
    兔多克隆抗体to Nucleobindin 1
  • 特异性ab23360 recognises Nucleobindin 1
  • 经测试应用适用于: WB, ICC, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide: PAAHPEGQLK FHPDTDDVPV PAPAGDQKEV DTSEKKLLER LPEVEVPQHL conjugated to KLH, corresponding to a region within C terminal amino acids 413-462 of Human Nucleobindin 1

  • 阳性对照
    • HeLa cells, HepG2

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab23360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 54 kDa.
ICC Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能Major calcium-binding protein of the Golgi. May have a role in calcium homeostasis.
  • 组织特异性Expressed both in fetal and adult heart, lung, liver, kidney and brain, and in adult skeletal muscle, placenta and pancreas.
  • 序列相似性Belongs to the nucleobindin family.
    Contains 2 EF-hand domains.
  • 结构域The EF-hand domains are unfolded in the absence of Ca(2+) and fold upon Ca(2+) addition.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Golgi apparatus > cis-Golgi network membrane. Cytoplasm. A small fraction of the protein may be cytoplasmic.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CALNUC antibody
    • FLJ40471 antibody
    • NUC antibody
    • NUCB 1 antibody
    • NUCB1 antibody
    • NUCB1_HUMAN antibody
    • Nucleobindin 1 antibody
    • Nucleobindin-1 antibody
    see all

Anti-Nucleobindin 1 antibody 图像

  • Golgi of HeLa cells are detected with ab23360 (green). Nuclei (red) were stained with DAPI.
  • Anti-Nucleobindin 1 antibody (ab23360) at 1 µg/ml + HepG2 lysates

    Predicted band size : 54 kDa

Anti-Nucleobindin 1 antibody (ab23360)参考文献

ab23360 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (MDA-MB-231 breast cancer cell line)
Specification MDA-MB-231 breast cancer cell line
Fixative Paraformaldehyde
Permeabilization Yes - Triton 0.03%
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 25°C
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提交于 Oct 09 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"