The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This peptide may be used for neutralization and control experiments with the polyclonal antibody that reacts with this product and N-CoR, catalog ab3482. Using a solution of peptide of equal volume and concentration to the corresponding antibody will yield a large molar excess of peptide (~ 70-fold) for competitive inhibition of antibody-protein binding reactions.
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
N Cor/SMRT corepressor Rip13
Nuclear receptor co repressor 1
Nuclear receptor corepressor 1
Retinoid X receptor interacting protein 13
thyroid hormone and retinoic acid receptor associated corepressor 1
功能Mediates transcriptional repression by certain nuclear receptors. Part of a complex which promotes histone deacetylation and the formation of repressive chromatin structures which may impede the access of basal transcription factors.
序列相似性Belongs to the N-CoR nuclear receptor corepressors family. Contains 2 SANT domains.
结构域The N-terminal region contains three independent domains that are capable of mediating transcriptional repression (RD1, RD2 and RD3). The C-terminal region contains two separate nuclear receptor-interacting domains (ID1 and ID2), each of which contains a conserved sequence referred to as the CORNR box. This motif is necessary and sufficient for binding to unligated nuclear hormone receptors, while sequences flanking the CORNR box determine the precise nuclear hormone receptor specificity.
翻译后修饰Ubiquitinated; mediated by SIAH2 and leading to its subsequent proteasomal degradation.