Anti-Nuclear Pore Complex Proteins抗体[414] (ab50008)

概述

  • 产品名称Anti-Nuclear Pore Complex Proteins抗体[414]
    参阅全部 Nuclear Pore Complex Proteins 一抗
  • 描述
    小鼠单克隆抗体[414] to核Pore Complex Proteins
  • 特异性ab50008recognizes several proteins in the nuclear pore complex.
  • 经测试应用适用于: WB, IP, Electron Microscopy, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Saccharomyces cerevisiae, Xenopus laevis
  • 免疫原

    rat liver nuclei extract

  • 阳性对照
    • HeLa total cell extract.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 克隆单克隆
  • 克隆编号414
  • 骨髓瘤NS1
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab50008 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
Electron Microscopy
ICC/IF
  • 应用说明Electron Microscopy: Use at an assay dependent dilution.
    ICC/IF: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    WB: Use at a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 106 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    Anti-Nuclear Pore Complex Proteins antibody [414] 图像

    • Lane 1 : Anti-Nuclear Pore Complex Proteins antibody [414] (ab50008) at 1 µg/ml
      Lane 2 : Negative control - Goat Anti-Mouse IgG only

      Lane 1 : Extract from HeLa cells
      Lane 2 : As above

      Secondary
      Lane 1 : Goat Anti-Mouse IgG


      Predicted band size : 106 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)
    • ab50008 (1:500, 1hr/ 37oC) staining Nuclear pore complex proteins in HeLa cells (green). Cells were fixed in PHEM buffer (45 mM Hepes pH 6.9, 45 mM Pipes pH 6.9, 5 mM MgCl2, 10 mM EGTA, 4% PFA) /0,2% Triton x-100, 10 min at room T°, then quickly wash 3 x in PBS. ) Good results are also obtained after straight PFA fix: fix in 3.7% PFA / 30 mM sucrose for 10 min at room T°, quickly wash 3 x in PBS, then incubatein PBS/ 0.1% TritonX-100 for 5 min and further in 0.1 M glycine for 10 min.Image kindly provided by R. Mangiacasale,M. Ciciarello and P. Lavia, Institute of. Molecular Biology and Pathology, CNR - Rome (Italy)

    Anti-Nuclear Pore Complex Proteins antibody [414] (ab50008)参考文献

    This product has been referenced in:
    • Light WH  et al. A conserved role for human Nup98 in altering chromatin structure and promoting epigenetic transcriptional memory. PLoS Biol 11:e1001524 (2013). Read more (PubMed: 23555195) »
    • Cubeñas-Potts C  et al. SENP1 and SENP2 affect spatial and temporal control of sumoylation in mitosis. Mol Biol Cell 24:3483-95 (2013). ICC/IF ; Human . Read more (PubMed: 24048451) »

    See all 7 Publications for this product

    Product Wall

    I am very pleased to hear you would like to accept our offer and test one or both of these products in blocking experiements. Each code is associated with a paricular antibody so be sure tho reference the correct code when ordering and submitting the A...

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    Thank you very much for your interest in our Nuclear Pore Complex Proteins antibodies. I have spoken to the labs about these product and have found that these are indeed the same clone detailed inin the papers by Davis & Blobel as we spoke of. Read More

    Thank you for your enquiry. I can confirm this antibody is sourced externally. The originator has confirmed that it will recognise several proteins in the nuclear pore complex in various species. These proteins range in size between 62 and 210 kDa....

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    Application Immunocytochemistry/ Immunofluorescence
    Sample Kangaroo Cell (rat kangaroo kidney epithelial cell line PtK2)
    Specification rat kangaroo kidney epithelial cell line PtK2
    Fixative Paraformaldehyde
    Permeabilization Yes - Triton X-100
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
    Username

    Siegfried Reipert

    Verified customer

    提交于 Sep 09 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"