Anti-Nuclear Pore Complex Proteins抗体[39C7] (ab60080)

概述

  • 产品名称
    Anti-Nuclear Pore Complex Proteins抗体[39C7]
    参阅全部 Nuclear Pore Complex Proteins 一抗
  • 描述
    小鼠单克隆抗体[39C7] to核Pore Complex Proteins
  • 经测试应用
    适用于: Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Saccharomyces cerevisiae
  • 免疫原

    Yeast nuclear preparation

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.1% Sodium azide
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    单克隆
  • 克隆编号
    39C7
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab60080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1-2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/500. 1/100 - 1/500 (yeast cells) and 1/50 - 1/100 (mammalian cells)

靶标

图片

  • Overlay histogram showing HeLa cells stained with ab60080 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab60080, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Hela cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

文献

This product has been referenced in:
  • Ljubojevic S  et al. Early remodeling of perinuclear Ca2+ stores and nucleoplasmic Ca2+ signaling during the development of hypertrophy and heart failure. Circulation 130:244-55 (2014). Human . Read more (PubMed: 24928680) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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