重组Anti-Nuclear Matrix蛋白p84抗体[EPR5662(2)] (ab131268)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5662(2)] to Nuclear Matrix Protein p84
- Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Nuclear Matrix蛋白p84抗体[EPR5662(2)]
参阅全部 Nuclear Matrix Protein p84 一抗 -
描述
兔单克隆抗体[EPR5662(2)] to核Matrix蛋白p84 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), ICC/IF, WB, IHC-Pmore details
不适用于: IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human Nuclear Matrix Protein p84 aa 350-450. The exact sequence is proprietary.
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阳性对照
- WB: HeLa, U-87 MG, 293T and HepG2 (ab7900) cell lysates. IHC-P: Human breast carcinoma tissue. ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR5662(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131268于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/120.
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ICC/IF |
1/100.
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WB | (1) |
1/10000 - 1/50000. Detects a band of approximately 84 kDa (predicted molecular weight: 76 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
(Heat to 98°C, allow to cool for 10-20 minutes) |
说明 |
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Flow Cyt (Intra)
1/120. |
ICC/IF
1/100. |
WB
1/10000 - 1/50000. Detects a band of approximately 84 kDa (predicted molecular weight: 76 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes) |
靶标
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功能
Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and THOC4.
Regulates transcriptional elongation of a subset of genes. Participates in an apoptotic pathway which is characterized by activation of caspase-6, increases in the expression of BAK1 and BCL2L1 and activation of NF-kappa-B. This pathway does not require p53/TP53, nor does the presence of p53/TP53 affect the efficiency of cell killing. Activates a G2/M cell cycle checkpoint prior to the onset of apoptosis. Apoptosis is inhibited by association with RB1. -
组织特异性
Ubiquitous. Expressed in various cancer cell lines. Expressed at very low levels in normal breast epithelial cells and highly expressed in breast tumors. Expression is strongly associated with an aggressive phenotype of breast tumors and expression correlates with tumor size and the metastatic state of the tumor progression. -
序列相似性
Contains 1 death domain. -
结构域
An intact death domain is needed for apoptosis. -
翻译后修饰
Expression is altered specifically during apoptosis and is accompanied by the appearance of novel forms with smaller apparent molecular mass. -
细胞定位
Cytoplasm and Nucleus speckle. Nucleus > nucleoplasm. Nucleus matrix. Cytoplasm. Can shuttle between the nucleus and cytoplasm. Nuclear localization is required for induction of apoptotic cell death. Translocates to the cytoplasm during the early phase of apoptosis execution. - Information by UniProt
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数据库链接
- Entrez Gene: 9984 Human
- Entrez Gene: 225160 Mouse
- Entrez Gene: 291797 Rat
- Omim: 606930 Human
- SwissProt: Q96FV9 Human
- SwissProt: Q8R3N6 Mouse
- SwissProt: P59924 Rat
- Unigene: 712543 Human
see all -
形式
Nuclear (Isoform 1) and Cytoplasmic (Isoform 1 and 2). -
别名
- hTREX84 antibody
- Death domain containing protein p84N5 antibody
- HPR 1 antibody
see all
图片
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All lanes : Anti-Nuclear Matrix Protein p84 antibody [EPR5662(2)] (ab131268) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : U-87 MG cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 76 kDa -
Immunofluorescent staining of HeLa cells labelling Nuclear Matrix Protein p84 with ab131268 at 1/100 dilution.
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Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue labeling Nuclear Matrix Protein p84 with ab131268 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Nuclear Matrix Protein p84 with purified ab131268 at 1/120 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab131268 被引用在 13 文献中.
- Sato T et al. Importance of the Q/N-rich segment for protein stability of endogenous mouse TDP-43. Sci Rep 12:14923 (2022). PubMed: 36056157
- Liu L et al. An RFC4/Notch1 signaling feedback loop promotes NSCLC metastasis and stemness. Nat Commun 12:2693 (2021). PubMed: 33976158
- Zhang P et al. CLCA2 suppresses the proliferation, migration and invasion of cervical cancer. Exp Ther Med 22:776 (2021). PubMed: 34055075
- Liu QQ et al. Amplification of DDR2 mediates sorafenib resistance through NF-κB/c-Rel signaling in hepatocellular carcinoma. Cell Biol Int 45:1906-1916 (2021). PubMed: 33969575
- Soutto M et al. NF-kB-dependent activation of STAT3 by H. pylori is suppressed by TFF1. Cancer Cell Int 21:444 (2021). PubMed: 34419066