Anti-Nuclear Matrix Protein p84抗体[5E10] (ab94812)

概述

  • 产品名称Anti-Nuclear Matrix Protein p84抗体[5E10]
    参阅全部 Nuclear Matrix Protein p84 一抗
  • 描述
    小鼠单克隆抗体[5E10] to核Matrix Protein p84
  • 特异性ab94812 recognizes an 84 kD nuclear matrix protein that was first discovered through its association with the N terminal region of retinoblastoma protein.
  • 经测试应用适用于: IP, ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Fusion protein, corresponding to amino acids 15 - 374 of Human Nuclear Matrix Protein p84, expressed in E. coli.

  • 阳性对照
    • Molt 4, HeLa and Raji cell lysates

性能

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应用

Our Abpromise guarantee covers the use of ab94812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use a concentration of 0.5 - 2 µg/ml.
ICC/IF Use a concentration of 0.5 - 2 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 84 kDa (predicted molecular weight: 76 kDa).

靶标

  • 功能Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and THOC4.
    Regulates transcriptional elongation of a subset of genes. Participates in an apoptotic pathway which is characterized by activation of caspase-6, increases in the expression of BAK1 and BCL2L1 and activation of NF-kappa-B. This pathway does not require p53/TP53, nor does the presence of p53/TP53 affect the efficiency of cell killing. Activates a G2/M cell cycle checkpoint prior to the onset of apoptosis. Apoptosis is inhibited by association with RB1.
  • 组织特异性Ubiquitous. Expressed in various cancer cell lines. Expressed at very low levels in normal breast epithelial cells and highly expressed in breast tumors. Expression is strongly associated with an aggressive phenotype of breast tumors and expression correlates with tumor size and the metastatic state of the tumor progression.
  • 序列相似性Contains 1 death domain.
  • 结构域An intact death domain is needed for apoptosis.
  • 翻译后修饰Expression is altered specifically during apoptosis and is accompanied by the appearance of novel forms with smaller apparent molecular mass.
  • 细胞定位Cytoplasm and Nucleus speckle. Nucleus > nucleoplasm. Nucleus matrix. Cytoplasm. Can shuttle between the nucleus and cytoplasm. Nuclear localization is required for induction of apoptotic cell death. Translocates to the cytoplasm during the early phase of apoptosis execution.
  • Information by UniProt
  • 数据库链接
  • 形式Nuclear (Isoform 1) and Cytoplasmic (Isoform 1 and 2).
  • 别名
    • hTREX84 antibody
    • Death domain containing protein p84N5 antibody
    • HPR 1 antibody
    • HPR1 antibody
    • hTREX84 antibody
    • Nuclear matrix protein p84 antibody
    • P84 antibody
    • p84N5 antibody
    • Tho 1 antibody
    • THO complex 1 antibody
    • THO complex subunit 1 antibody
    • Tho1 antibody
    • THOC 1 antibody
    • Thoc1 antibody
    • THOC1_HUMAN antibody
    see all

Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab94812)参考文献

ab94812 has not yet been referenced specifically in any publications.

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