Anti-Nuclear Factor Erythroid 2 Related Factor 1抗体(ab90524)

概述

  • 产品名称
    Anti-Nuclear Factor Erythroid 2 Related Factor 1抗体
    参阅全部 Nuclear Factor Erythroid 2 Related Factor 1 一抗
  • 描述
    兔多克隆抗体to核Factor Erythroid 2 Related Factor 1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog
  • 免疫原

    Synthetic peptide designed within residues: TYNMAPSALD SADLPPPSAL KKGSKEKQAD FLDKQMSRDE HRARAMKIPF, corresponding to internal amino acids 576 - 625 of Human Nuclear Factor Erythroid 2 Related Factor 1 (NP_003195).

  • 阳性对照
    • Jurkat cell lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab90524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 85 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

图片



  • Predicted band size : 85 kDa
    Western blot analysis of HepG2 cell lysate labeling Nuclear Factor Erythroid 2 Related Factor 1 with ab90524 at 1.0µg/ml.
  • Anti-Nuclear Factor Erythroid 2 Related Factor 1 antibody (ab90524) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 85 kDa

实验方案

文献

ab90524 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for your reply.

Please find the information below regarding the testing discount offer, to test ab90425 in ChIP. Also I have sent you ab90425 as a free of charge replacement for ab34682 which was mislabeled on our website. There sho...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (A549 cells)
Loading amount
10000 cells
Specification
A549 cells
Treatment
26 nM DMSO/bortezomib (BTZ) 24 hours
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 03 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册