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Nuclear Extraction Kit (ab113474) provides a simple and selective method along with all necessary reagents for nuclear protein extraction / nuclear protein fractionation in just 1 hour for a variety of applications such as western blotting, protein-DNA binding assays, nuclear enzyme assays or any other procedures requiring optimized nuclear proteins. The protocol is fast and easy-to-use, and isolates very abundant yields of nuclear extract from mammalian cells or tissue samples.
We recommend our alternative Soluble / Insoluble Nuclear Extraction kit to extract separate fractions of soluble and insoluble proteins.
Not sure if this is the right product for you? Check out our EpiSeeker Sample Preparation Guide for help.
Compared to other kits that use conventional nuclear extraction / nuclear fractionation methods, the buffers included in ab113474 contain much lower amounts of salts (80% less than conventional kits) and no SDS, which allows much better retention of enzyme activity in the nuclear extracts.
|1000X Protease Inhibitor Cocktail||1 x 110µl|
|10X Pre-Extraction Buffer||1 x 10ml|
|DTT Solution (1000X)||1 x 110µl|
|ENE2 (Extraction Buffer)||1 x 10ml|
Our Abpromise guarantee covers the use of ab113474 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"