概述

  • 产品名称
  • 样品类型
    Tissue, Adherent cells, Suspension cells
  • 检测时间
    1h 00m
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Mammal
  • 产品概述

    Nuclear Extraction Kit (ab113474) provides a simple and selective method along with all necessary reagents for extracting nuclear proteins in just 1 hour for a variety of applications such as western blotting, protein-DNA binding assays, nuclear enzyme assays or any other procedures requiring optimized nuclear proteins. The protocol is fast and easy-to-use, and also isolates very abundant yields of nuclear extract from mammalian cells or tissue samples.


    We recommend our alternative Soluble / Insoluble Nuclear Extraction kit to extract separate fractions of soluble and insoluble proteins.


    Not sure if this is the right product for you? Check out our EpiSeeker Sample Preparation Guide for help.


    Compared to other kits that use conventional nuclear extraction methods, the buffers included in ab113474 contain much lower amounts of salts (80% less than conventional kits) and no SDS, which would allow the enzyme activity to be better kept in the nuclear extracts.

  • 说明

     

     

  • 经测试应用
    适用于: Functional Studiesmore details

性能

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    应用

    Our Abpromise guarantee covers the use of ab113474 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Functional Studies Use at an assay dependent concentration.

    Nuclear Extraction Kit 图像

    • Nuclear extracts were prepared from MCF-7 cells and the activity of HDACs were measured using different amounts of the extract. The result shown in the figure demonstrates the ab113474's high specificity.

    实验方案

    Nuclear Extraction Kit (ab113474)参考文献

    This product has been referenced in:
    • O'Leary VB  et al. Long non-coding RNA PARTICLE bridges histone and DNA methylation. Sci Rep 7:1790 (2017). Functional Studies ; Human . Read more (PubMed: 28496150) »
    • Appu AP  et al. Increasing N-acetylaspartate in the Brain during Postnatal Myelination Does Not Cause the CNS Pathologies of Canavan Disease. Front Mol Neurosci 10:161 (2017). Read more (PubMed: 28626388) »

    See all 26 Publications for this product

    Product Wall

    Yes, this kit can be definitely used for frozen tissues. The lab confirmed it.

    Thank you for your inquiry.

    We have two Nuclear Extraction Kits in our catalog at the moment:

    ab113474

    http://www.abcam.com/index.html?datasheet=113474 (or use the following: http://www.abcam.com/index.html?datasheet=11347...

    Read More

    Yes, the cytosolic fraction when using ab113474 Nuclear Extraction Kit includes membrane proteins.

    Thank you for the follow up message.


    I can confirm that the concentration of DTT included in the kit is 1M.


    I hope this will be helpful. If you have any further questions, please do not hesitate to contact me.

    This kit will work better with 2 million cells however you can still use this with half a million cells, when using with 500,000 cells please keep the volume of buffers and reagents proportional to 1 million cells.

    We recommend using Bradford ...

    Read More



    I contacted the laboratories. They confirmed that PIC is not required for the tissue homogenization preparation protocol as the active proteases in tissue are much less than that in cultured cells.



    I can confirm that if the cytosol is not required for much consideration, DTT can be excluded in pre-lysis buffer too.



    Yes you can use ab113474, EpiSeeker Nuclear Extraction Kit, to purify soluble nuclear proteins from tissue culture cells or tissues and then detect HIF1 alpha in that extract using ab133104, HIF-1 alpha Transcription Factor Assay Kit.

    A rotor-stator homogenizer even at low speed may disrupt the nuclear membrane. We recommend using a Dounce homogenizer for isolating intact nuclei.

    Nuclear Protein extraction and Histone protein extraction from cell samples frozen at -80oC could still work using ab113474 and ab113476. However, the enzyme activity of nuclear extracts from frozen tissues may be much lower than that from fresh tissues.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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