概述

  • 产品名称核Apoptosis Assay试剂盒
  • 样品类型
    Adherent cells, Suspension cells
  • 种属反应性
    与反应: Human
  • 产品概述

    ab112151 is designed to monitor cell apoptosis by measuring the apoptotic chromatin condensation. The compacted chromatin of apoptotic cells binds higher amounts of nuclear dye compared to the healthy cells. The fluorometric assay is based on the detection of the DNA contents in cells using our proprietary non-fluorescent dye that becomes strongly fluorescent upon binding to cellular DNA.

    In normal cells, Nuclear Green DCS1 Dye is not cell permeable. However, in apoptotic cells, Nuclear Green DCS1 Dye can easily get into the cells with compromised plasma membranes. Once inside the cell, the dye binds to intracellular DNA producing highly fluorescent complexes which identify the cells as non-viable cells. The staining density with Nuclear Green DCS1 Dye can be measured with a flow cytometer at Ex/Em = 490/520 nm (FL1 channel) or a fluorescence microscope (FITC filter set).


    ab112151 can be used together with our other apoptosis reagents, such as our ab112149 NIR Mitochondrial Membrane Potential Assay Kit, for multi-parametric study of cell viability and apoptosis. The kit is optimized for screening apoptosis activators and inhibitors.

    Visit our FAQs page for tips and troubleshooting.

  • 说明

    Store ab112151 dessicated.

  • 经测试应用适用于: FM, Functional Studies, Flow Cytmore details

性能

    应用

    Our Abpromise guarantee covers the use of ab112151 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    FM Use at an assay dependent concentration.
    Functional Studies Use at an assay dependent concentration.
    Flow Cyt Use at an assay dependent concentration.

    Nuclear Apoptosis Assay Kit 图像

    • The increase in fluorescence intensity of ab112151 DCS1 Dye with the addition of Camptothecin in Jurkat cells. Jurkat cells were untreated (A) or treated overnightwith 20 μM camptothecin (B) in a 37 oC, 5% CO2 incubator, and then dye loaded with ab112151 DCS1 Dye for 60 minutes.

    实验方案

    Nuclear Apoptosis Assay Kit (ab112151)参考文献

    ab112151 has not yet been referenced specifically in any publications.

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