Mouse, Rat, Chicken, Cow
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human NUCKS1.
(Peptide available as
This antibody gave a positive signal in HepG2 whole cell lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This antibody gave a positive result in ELISA against the immunizing peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Phosphorylated by CDK1 and casein kinase. Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
Western blot - NUCKS1 antibody (ab77770)
Anti-NUCKS1 antibody (ab77770) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution Developed using the ECL technique Performed under reducing conditions. Predicted band size : 27 kDa Observed band size : 27 kDa Additional bands at : 24 kDa. We are unsure as to the identity of these extra bands. Exposure time : 20 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NUCKS1 antibody (ab77770)
IHC image of NUCKS1 staining in Human Normal Kidney FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77770, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
has not yet been referenced specifically in any publications.
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