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Our Abpromise guarantee covers the use of ab123726 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||1/100 - 1/500. Predicted molecular weight: 61 kDa.|
ab123726 staining NTN3 in tumor xenograft in nude mouse with human breast cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked for 10 minutes at 37°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/50) for 30 minutes at 37°C. An undiluted HRP polymer-conjugated goat anti-rabbit IgG monoclonal was used as the secondary antibody.
ab123726 has not yet been referenced specifically in any publications.