Anti-NTH1抗体[2660C1a] (ab70726)

概述

  • 产品名称Anti-NTH1抗体[2660C1a]
    参阅全部 NTH1 一抗
  • 描述
    小鼠单克隆抗体[2660C1a] to NTH1
  • 经测试应用适用于: ICC/IF, WB, Dot blotmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant N-terminal fragment of human NTH1

  • 阳性对照
    • Recombinant human NTH1. Whole cell lysates from human HEK293, mouse NIH3T3 and rat F2408 cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明ab70726 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
  • 克隆单克隆
  • 克隆编号2660C1a
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab70726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 0.2 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa).
Dot blot Use at an assay dependent dilution.

靶标

  • 功能Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions.
  • 组织特异性Widely expressed with highest levels in heart and lowest levels in lung and liver.
  • 序列相似性Belongs to the Nth/MutY family.
  • 发展阶段Expression levels are regulated during the cell cycle with increased levels during early and mid S-phase.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Bifunctional DNA N glycoslyase/DNA (apurinic or apyrimidinic site) lyase antibody
    • DNA glycoslyase/AP lyase antibody
    • Endonuclease III like protein 1 antibody
    • Endonuclease III-like protein 1 antibody
    • hNTH1 antibody
    • NTH 1 antibody
    • nth endonuclease III like 1 (E. coli) antibody
    • NTH endonuclease III Like 1 antibody
    • NTH1 antibody
    • NTHL 1 antibody
    • Nthl1 antibody
    • NTHL1_HUMAN antibody
    • OCTS 3 antibody
    • OCTS3 antibody
    see all

Anti-NTH1 antibody [2660C1a] 图像

  • All lanes : Anti-NTH1 antibody [2660C1a] (ab70726) at 0.2 µg/ml

    Lane 1 : Whole cell lysate from human HEK293 cells
    Lane 2 : Whole cell lysate from mouse NIH3T3 cells
    Lane 3 : Whole cell lysate from rat F2408 cells

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 34 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-NTH1 antibody [2660C1a] (ab70726) at 0.2 µg/ml + immunised recombinant human NTH1 protein

    Predicted band size : 34 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • ab70726 staining NTH1 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 2% BSA for 45 minutes at room temperature. Samples were incubated with primary antibody (1/300 in PBS + 2% BSA) for 14 hours at 4°C. An Alexa Fluor® 594-conjugated goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

Anti-NTH1 antibody [2660C1a] (ab70726)参考文献

ab70726 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 2% · Temperature: RT°C
Sample Human Cell (HeLa cells)
Specification HeLa cells
Permeabilization Yes - 0.2% Triton-X100
Fixative Paraformaldehyde
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Verified customer

提交于 May 15 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Loading amount 40 µg
Specification Mouse embryonic fibroblasts
Gel Running Conditions Reduced Denaturing (4-20% Tris-Gly gel)
Blocking step LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
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Abcam user community

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提交于 Apr 09 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 20 µg
Specification HeLa cells
Gel Running Conditions Reduced Denaturing (4-16% Tris-Gly gel)
Blocking step LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Username

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Verified customer

提交于 Apr 09 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"