概述

  • 产品名称Anti-NTE抗体
    参阅全部 NTE 一抗
  • 描述
    小鼠单克隆抗体to NTE
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Recombinant Fragment
    预测可用于: Mouse, Cow, Human, Orangutan
  • 免疫原

    Recombinant fragment: ESRRADVLAF PSSGFTDLAE IVSRIEPPTS YVSDGCADGE ESDCLTEYEE DAGPDCSRDE GGSPEGASPS TASEMEEEKS ILRQRRCLPQ EPPGSATDA, corresponding to amino acids 1229-1327 of Human NTE with a tag of 26 kDa.

性能

应用

Our Abpromise guarantee covers the use of ab77153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明ELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 150 kDa.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.



    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Phospholipase B that deacylates intracellular phosphatidylcholine (PtdCho), generating glycerophosphocholine (GroPtdCho). This deacylation occurs at both sn-2 and sn-1 positions of PtdCho. Its specific chemical modification by certain organophosphorus (OP) compounds leads to distal axonopathy.
    • 组织特异性Expressed in brain, placenta, kidney, neuron and skeletal muscle.
    • 疾病相关Defects in PNPLA6 are the cause of spastic paraplegia autosomal recessive type 39 (SPG39) [MIM:612020]; also known as NTE-related motor neuron disorder (NTEMND). Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. SPG39 is associated with a motor axonopathy affecting upper and lower limbs and resulting in progressive wasting of distal upper and lower extremity muscles.
    • 序列相似性Belongs to the NTE family.
      Contains 3 cyclic nucleotide-binding domains.
      Contains 1 patatin domain.
    • 翻译后修饰Glycosylated.
    • 细胞定位Endoplasmic reticulum membrane. Anchored to the cytoplasmic face of the endoplasmic reticulum by its amino-terminal transmembrane segment.
    • Information by UniProt
    • 数据库链接
    • 别名
      • EC 3.1.1.5 antibody
      • Neuropathy target esterase antibody
      • NTEMND antibody
      • Patatin like phospholipase domain containing 6 antibody
      • Patatin like phospholipase domain containing protein 6 antibody
      • Patatin-like phospholipase domain-containing protein 6 antibody
      • PLPL6_HUMAN antibody
      • Pnpla6 antibody
      • SPG39 antibody
      • sws antibody
      see all

    Anti-NTE antibody 图像

    • Anti-NTE antibody (ab77153) at 5 µg/ml + immunogen at 0.2 µg

      Secondary
      Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution
      Developed using the ECL technique

      Predicted band size : 150 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)

    Anti-NTE antibody (ab77153)参考文献

    ab77153 has not yet been referenced specifically in any publications.

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