Mouse, Rat, Pig
Recombinant fragment, corresponding to a region within amino acids 1-246 of Human NTAN1 (AAH17336).
293T and Raji whole cell lysates; Human hepatoma tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol, Glycine, 1.21% Tris
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Immunogen affinity purified
ab111710 is purified by antigen-affinity chromatography.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 35 kDa.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer ( PH8.0)
NTAN1 is required for the ubiquitin-dependent turnover of intracellular proteins that initiate with Met-Asn. This enzyme does not act on substrates with internal or C-terminal asparagines and does not act on glutamine residues in any position.
N terminal Asn amidase antibody
N terminal asparagine amidase antibody
N terminal asparagine amidohydrolase antibody
Western blot - Anti-NTAN1 antibody (ab111710)
Anti-NTAN1 antibody (ab111710) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 35 kDa 10% SDS-PAGE.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NTAN1 antibody (ab111710)
ab111710 at 1/500 dilution staining NTAN1 in Formalin-fixed, paraffin-embedded Human Hepatoma tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"