概述

  • 产品名称Anti-NTAL抗体[NAP-07]
    参阅全部 NTAL 一抗
  • 描述
    小鼠单克隆抗体[NAP-07] to NTAL
  • 特异性The antibody reacts with Non-T cell activation linker (NTAL) a 30 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B cells, NK cells and myeloid cells.
  • 经测试应用适用于: WB, IP, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 91-244 of Human NTAL.

  • 阳性对照
    • RAMOS human lymphoma cell lysate, Raji human lymphoma cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab3992 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 µg/ml. Use under non reducing condition. (see Tkaczyk et al).
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent dilution.

靶标

  • 功能Involved in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. May also be involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and FCGR1 (high affinity immunoglobulin gamma Fc receptor I)-mediated signaling in myeloid cells. Couples activation of these receptors and their associated kinases with distal intracellular events through the recruitment of GRB2.
  • 组织特异性Highly expressed in spleen, peripheral blood lymphocytes, and germinal centers of lymph nodes. Also expressed in placenta, lung, pancreas and small intestine. Present in B-cells, NK cells and monocytes. Absent from T-cells (at protein level).
  • 疾病相关Note=LAT2 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of LAT2 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
  • 翻译后修饰Phosphorylated on tyrosines following cross-linking of BCR in B-cells, FCGR1 in myeloid cells, or FCER1 in mast cells; which induces the recruitment of GRB2.
    May be polyubiquitinated.
  • 细胞定位Cell membrane. Present in lipid rafts.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HSPC046 antibody
    • LAB antibody
    • LAT2 antibody
    • Linker for activation of B cells antibody
    • Linker for activation of B-cells antibody
    • Linker for activation of T cells family member 2 antibody
    • Linker for activation of T cells transmembrane adaptor 2 antibody
    • Linker for activation of T-cells family member 2 antibody
    • Membrane associated adapter molecule antibody
    • Membrane-associated adapter molecule antibody
    • Non T cell activation linker antibody
    • Non-T-cell activation linker antibody
    • NTAL_HUMAN antibody
    • WBSCR 5 antibody
    • Wbscr15 antibody
    • Williams Beuren syndrome chromosome region 15 antibody
    • Williams Beuren syndrome chromosome region 5 antibody
    • Williams-Beuren syndrome chromosomal region 15 protein antibody
    • Williams-Beuren syndrome chromosomal region 5 protein antibody
    • WSCR5 antibody
    see all

Anti-NTAL antibody [NAP-07] 图像

  • Subcellular localization of NTAL by confocal microscopy in the myeloid cell line THP-1. THP-1 cells were permeabilized and immunostained using antibody NAP-07 (green). Nuclei are vizualized by propidium iodide (red).

Anti-NTAL antibody [NAP-07] (ab3992)参考文献

This product has been referenced in:
  • Malhotra S  et al. Vav and Rac activation in B cell antigen receptor endocytosis involves Vav recruitment to the adapter protein LAB. J Biol Chem 284:36202-12 (2009). WB ; Mouse . Read more (PubMed: 19858206) »
  • Mutch CM  et al. Activation-induced endocytosis of the raft-associated transmembrane adaptor protein LAB/NTAL in B lymphocytes: evidence for a role in internalization of the B cell receptor. Int Immunol 19:19-30 (2007). Read more (PubMed: 17090619) »

See all 8 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - other (RAW264.7)
Loading amount 40 µg
Specification RAW264.7
Gel Running Conditions Non-reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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提交于 Oct 24 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"