概述

  • 产品名称Anti-NTAL抗体
    参阅全部 NTAL 一抗
  • 描述
    兔多克隆抗体to NTAL
  • 特异性This polyclonal antibody reacts with NTAL, a 30 kDa transmembrane adaptor protein expressed in membrane microdomains (rafts) of B cells, NK cells and myeloid cells. We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 91-244 of Human NTAL

  • 阳性对照
    • RAMOS human lymphoma cell line, THP-1 human monocytic leukemia cell line. Negative control for WB: Jurkat human leukemia T-cell line.

性能

应用

Our Abpromise guarantee covers the use of ab36451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: Use at a concentration of 1 µg/ml (incubation 60 min at RT; or overnight at 4°C). Sample preparation: Lysis buffer with 1% laurylmaltoside. Use under non reducing conditions. Detects a band of approximately 30 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Involved in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. May also be involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and FCGR1 (high affinity immunoglobulin gamma Fc receptor I)-mediated signaling in myeloid cells. Couples activation of these receptors and their associated kinases with distal intracellular events through the recruitment of GRB2.
    • 组织特异性Highly expressed in spleen, peripheral blood lymphocytes, and germinal centers of lymph nodes. Also expressed in placenta, lung, pancreas and small intestine. Present in B-cells, NK cells and monocytes. Absent from T-cells (at protein level).
    • 疾病相关Note=LAT2 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of LAT2 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
    • 翻译后修饰Phosphorylated on tyrosines following cross-linking of BCR in B-cells, FCGR1 in myeloid cells, or FCER1 in mast cells; which induces the recruitment of GRB2.
      May be polyubiquitinated.
    • 细胞定位Cell membrane. Present in lipid rafts.
    • Information by UniProt
    • 数据库链接
    • 别名
      • HSPC046 antibody
      • LAB antibody
      • LAT2 antibody
      • Linker for activation of B cells antibody
      • Linker for activation of B-cells antibody
      • Linker for activation of T cells family member 2 antibody
      • Linker for activation of T cells transmembrane adaptor 2 antibody
      • Linker for activation of T-cells family member 2 antibody
      • Membrane associated adapter molecule antibody
      • Membrane-associated adapter molecule antibody
      • Non T cell activation linker antibody
      • Non-T-cell activation linker antibody
      • NTAL_HUMAN antibody
      • WBSCR 5 antibody
      • Wbscr15 antibody
      • Williams Beuren syndrome chromosome region 15 antibody
      • Williams Beuren syndrome chromosome region 5 antibody
      • Williams-Beuren syndrome chromosomal region 15 protein antibody
      • Williams-Beuren syndrome chromosomal region 5 protein antibody
      • WSCR5 antibody
      see all

    Anti-NTAL antibody 图像

    • All lanes : Anti-NTAL antibody (ab36451) at 1 µg/ml

      Lane 1 : NTAL-transfected HEK-293 FT cells
      Lane 2 : Negative control: Jurkat human leukemia T-cell line

      Secondary
      Lane 2 : goat anti-rabbit antibody


      Performed under non-reducing conditions.

      Observed band size : 30 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 58 kDa (possible non-specific binding),65 kDa (possible non-specific binding),70 kDa (possible non-specific binding).

    Anti-NTAL antibody (ab36451)参考文献

    ab36451 has not yet been referenced specifically in any publications.

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