Synthetic peptide derived from an internal region of human NT5C1A.
- Extracts from MCF-7 cells and HepG2 cells. A549 cells.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
||1/500 - 1/1000.
Dephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides and has a broad substrate specificity. Helps to regulate adenosine levels in heart during ischemia and hypoxia.
Highly expressed in skeletal muscle. Detected at intermediate levels in heart, brain, kidney and pancreas.
Belongs to the 5'-nucleotidase type 3 family.
Information by UniProt
- 5' nucleotidase cytosolic IA antibody
- 5NT1A_HUMAN antibody
- AMP specific 5' NT antibody
Anti-NT5C1A antibody 图像
Western blot - NT5C1A antibody (ab75101)
All lanes : Anti-NT5C1A antibody (ab75101) at 1/500 dilution
Lane 1 : Extracts from MCF-7 cells
Lane 2 : Extracts from HepG2 cells
Lane 3 : Extracts from MCF-7 cells with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 41 kDa
Observed band size : 41 kDa
Additional bands at : 26 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - NT5C1A antibody (ab75101)
Immunofluorescence analysis of NT5C1A in A549 cells using ab75101, at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
Anti-NT5C1A antibody (ab75101)参考文献
has not yet been referenced specifically in any publications.
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