Anti-NSUN5抗体(ab121633)

概述

  • 产品名称Anti-NSUN5抗体
    参阅全部 NSUN5 一抗
  • 描述
    兔多克隆抗体to NSUN5
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence: QLPRFVRVNT LKTCSDDVVD YFKRQGFSYQ GRASSLDDLR ALKGKHFLLD PLMPELLVFP AQTDLHEHPL YRAGHLIL, corresponding to amino acids 133-210 of Human NSUN5.

  • 阳性对照
    • U 251 MG lysate; Human rectum tissue

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab121633 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

靶标

  • 功能May have S-adenosyl-L-methionine-dependent methyl-transferase activity.
  • 组织特异性Ubiquitous. Detected in placenta, heart and skeletal muscle.
  • 疾病相关Note=NSUN5 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
  • 序列相似性Belongs to the methyltransferase superfamily. RsmB/NOP family.
  • 翻译后修饰Isoform 2 is phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ10267 antibody
    • MGC986 antibody
    • NOL1 antibody
    • NOL1-related protein antibody
    • NOL1/NOP2/Sun domain family member 5 antibody
    • NOL1R antibody
    • NOP2/Sun domain family, member 5 antibody
    • NOP2/Sun domain family, member 5A antibody
    • NSUN5 antibody
    • NSUN5_HUMAN antibody
    • p120 antibody
    • Putative methyltransferase NSUN5 antibody
    • WBSCR20 antibody
    • WBSCR20A antibody
    • Williams Beuren syndrome chromosome region 20A antibody
    • Williams-Beuren syndrome chromosomal region 20A protein antibody
    • Williams-Beuren syndrome critical region protein 20 copy A antibody
    see all

Anti-NSUN5 antibody 图像

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus and nucleoli. Recommended concentration of ab121633 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-NSUN5 antibody (ab121633) at 1/250 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human Plasma lysate
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

    Developed using the ECL technique
  • ab121633 at 1/250 dilution staining NSUN5 in Paraffin-embedded Human rectum tissue by Immunohistochemistry.

Anti-NSUN5 antibody (ab121633)参考文献

ab121633 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (mESCs)
Gel Running Conditions Non-reduced Denaturing (4-20)
Loading amount 30 µg
Specification mESCs
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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提交于 Jan 06 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"