Anti-NSP 5 alpha 3 alpha抗体(ab87801)

概述

  • 产品名称
    Anti-NSP 5 alpha 3 alpha抗体
    参阅全部 NSP 5 alpha 3 alpha 一抗
  • 描述
    兔多克隆抗体to NSP 5 alpha 3 alpha
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region between residues 450 and 500 of human NSP 5 alpha 3 alpha (NP_001028725.1).

  • 阳性对照
    • Whole cell lysate from HeLa cells

性能

应用

Our Abpromise guarantee covers the use of ab87801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明
    IP: Use at 2 - 5 µg/mg of lysate.
    WB: 1/2000 - 1/10,000. Predicted molecular weight: 119 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 组织特异性
      Highly expressed in testis. Barely detectable in other tissues. Also highly expressed in some cancer cell lines.
    • 疾病相关
      Note=A chromosomal aberration involving CYTSB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with PDGFRB.
    • 序列相似性
      Belongs to the cytospin-A family.
      Contains 1 CH (calponin-homology) domain.
    • 细胞定位
      Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Cytokinesis and spindle organization B antibody
      • Cytospin B antibody
      • Cytospin-B antibody
      • CYTSB antibody
      • CYTSB_HUMAN antibody
      • FLJ36955 antibody
      • HCMOGT 1 antibody
      • HCMOGT antibody
      • HCMOGT-1 antibody
      • NSP antibody
      • NSP5 antibody
      • Nuclear structure protein 5 antibody
      • Specc1 antibody
      • Spectrin domain with coiled coils 1 antibody
      • Sperm antigen HCMOGT 1 antibody
      • Sperm antigen HCMOGT-1 antibody
      • Sperm antigen with calponin homology and coiled coil domains 1 antibody
      • Sperm antigen with calponin homology and coiled-coil domains 1 antibody
      • sperm antigen with calponin-like and coiled coil domains 1 antibody
      • Structure protein NSP5a3a antibody
      • Structure protein NSP5a3b antibody
      • Structure protein NSP5b3a antibody
      • Structure protein NSP5b3b antibody
      see all

    图片

    • All lanes : Anti-NSP 5 alpha 3 alpha antibody (ab87801) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg


      Predicted band size : 119 kDa
      Observed band size : 119 kDa


      Exposure time : 30 seconds
    • Detection of NSP 5 alpha 3 alpha in immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab87801 at 3 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot.

      Detection: Chemiluminescence with an exposure time of 10 seconds.

    文献

    ab87801 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Heart, liver, muscle)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Loading amount
    50 µg
    Specification
    Heart, liver, muscle
    Blocking step
    Licor Odyssey buffer with Tween as blocking agent for 30 minute(s) · Concentration: 0.001% · Temperature: 25°C
    Username

    Dr. Gillian Hunter

    Verified customer

    提交于 Nov 28 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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