Recombinant fragment, corresponding to a region within amino acids 1-245 of Human NSL1 (NP_056286).
- A431, H1299, HeLaS3, HepG2 whole cell lysate for WB. HeLa cells for IF.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/3000. Predicted molecular weight: 32 kDa.
||1/100 - 1/200.
Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Nucleus. Chromosome > centromere > kinetochore. Associated with the kinetochore.
Information by UniProt
- 4833432M17Rik antibody
- AI451597 antibody
- C1orf48 antibody
Western blot - Anti-NSL1 antibody (ab96728)
Anti-NSL1 antibody (ab96728) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 32 kDa
12% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-NSL1 antibody (ab96728)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96728 at 1/200 dilution (image 1) and merged with DNA probe (image 2).
has not yet been referenced specifically in any publications.
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