概述

  • 产品名称Anti-NSL1抗体
    参阅全部 NSL1 一抗
  • 描述
    兔多克隆抗体to NSL1
  • 经测试应用适用于: IHC-P, IPmore details
    不适用于: WB
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region between amino acids 150-200 of Human NSL1 (NP_056286.2)

  • 阳性对照
    • Whole cell lysate from HeLa cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7 to 8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab70835 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • 应用说明Is unsuitable for WB.
  • 靶标

    • 功能Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis.
    • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位Nucleus. Chromosome > centromere > kinetochore. Associated with the kinetochore.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 4833432M17Rik antibody
      • AI451597 antibody
      • C1orf48 antibody
      • DC31 antibody
      • DC8 antibody
      • DKFZp566O1646 antibody
      • Gm105 antibody
      • Gm984 antibody
      • Kinetochore associated protein NSL1 homolog antibody
      • Kinetochore-associated protein NSL1 homolog antibody
      • MIS14 antibody
      • NSL1 antibody
      • NSL1, MIND kinetochore complex component, homolog (S. cerevisiae) antibody
      • NSL1_HUMAN antibody
      see all

    Anti-NSL1 antibody 图像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling NSL1 with ab70835 at 1/1000 (1µg/ml). Detection: DAB.
    • Detection of Human NSL1 by Western Blot of Immunoprecipitates in Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells.
      Lane 1: ab70835 used for IP at 3 µg/mg lysate.
      Lane 2: immunoprecipitation with an other antibody
      Lane 3: ab70836 used for IP at 3 µg/mg lysate
      Lane 4: Control IgG IP
      Detection: Chemiluminescence with an exposure time of 3 minutes. For blotting ab70836 was used at 1 µg/ml.

    Anti-NSL1 antibody (ab70835)参考文献

    ab70835 has not yet been referenced specifically in any publications.

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