概述

  • 产品名称
  • 描述
    兔多克隆抗体to NSE2
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human NSE2.

    (Peptide available as ab94387.)

  • 阳性对照
    • This antibody gave a positive signal in the following human whole cell lysates: Caco2; HepG2; A431.

性能

应用

Our Abpromise guarantee covers the use of ab64683 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 28 kDa).

靶标

  • 功能
    E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. Acts as a E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TRAX, and maybe the cohesin components RAD21 and STAG2. SUMO protein-ligase activity is required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair.
  • 通路
    Protein modification; protein sumoylation.
  • 序列相似性
    Belongs to the NSE2 family.
    Contains 1 SP-RING-type zinc finger.
  • 翻译后修饰
    Sumoylated, possibly via autosumoylation.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C8orf36 antibody
    • E3 SUMO-protein ligase NSE2 antibody
    • hMMS21 antibody
    • hNSE2 antibody
    • methyl methanesulfonate sensitivity gene 21 antibody
    • MMS21 antibody
    • MMS21 homolog antibody
    • non-SMC element 2 antibody
    • Non-SMC element 2 homolog antibody
    • Non-structural maintenance of chromosomes element 2 homolog antibody
    • NSE2_HUMAN antibody
    • Nsmce2 antibody
    see all

图片

  • All lanes : Anti-NSE2 antibody (ab64683) at 1 µg/ml

    Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
    Lane 4 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 6 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 28 kDa
    Observed band size: 56 kDa (why is the actual band size different from the predicted?)


    Exposure time: 20 minutes


    Human E3 SUMO-protein ligase NSE2 contains a potential sumoylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.

实验方案

文献

ab64683 has not yet been referenced specifically in any publications.

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