NSE overexpression 293T裂解液(全细胞) (ab94096)

概述

  • 产品名称NSE overexpression 293T裂解液(全细胞)
  • 描述
    NSE overexpression裂解液
  • 常规说明ab94096 is a 293T cell transfected lysate in which Human NSE has been transiently over-expressed using a pCMV-NSE plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • 经测试应用适用于: WBmore details

性能

  • 背景Developmental stage: During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells. Function: Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival. Pathway: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5. Similarity: Belongs to the enolase family. Tissue specificity: The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

应用

Our Abpromise guarantee covers the use of ab94096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

NSE overexpression 293T lysate (whole cell) 图像

  • ab94096 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-NSE antibody (ab89428) at 1/500 dilution

    Lane 1 : NSE overexpression 293T lysate (whole cell) (ab94096)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

NSE overexpression 293T lysate (whole cell) (ab94096)参考文献

ab94096 has not yet been referenced specifically in any publications.

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