Anti-NSE抗体[EPR3377] (ab79757)




Our Abpromise guarantee covers the use of ab79757 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/250.
WB 1/5000 - 1/20000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
Flow Cyt 1/20 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.


  • 功能Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
  • 组织特异性The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
  • 通路Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
  • 序列相似性Belongs to the enolase family.
  • 发展阶段During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
  • 细胞定位Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2 phospho D glycerate hydrolyase antibody
    • 2-phospho-D-glycerate hydro-lyase antibody
    • Eno 2 antibody
    • ENO2 antibody
    • ENOG antibody
    • ENOG_HUMAN antibody
    • Enolase 2 (gamma, neuronal) antibody
    • Enolase 2 antibody
    • Enolase 2 gamma neuronal antibody
    • Enolase2 antibody
    • Epididymis secretory protein Li 279 antibody
    • Gamma enolase antibody
    • Gamma-enolase antibody
    • HEL S 279 antibody
    • Neural enolase antibody
    • Neuron specific enolase antibody
    • Neuron specific gamma enolase antibody
    • Neuron-specific enolase antibody
    • Neurone specific enolase antibody
    • NSE antibody
    see all

Anti-NSE antibody [EPR3377] 图像

  • Immunohistochemical analysis of paraffin-embedded human brain tissue using 1/250-1/500 ab79757.
  • ICC/IF image of ab79757 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab79757 at 1/25 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Overlay histogram showing HeLa cells stained with ab79757 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab79757, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized with 0.1% PBS-Tween 20 used under the same conditions.

  • All lanes : Anti-NSE antibody [EPR3377] (ab79757) at 1/20000 dilution

    Lane 1 : fetal brain lysate
    Lane 2 : SH-SY-5Y lysate
    Lane 3 : HeLa lysate
    Lane 4 : Y79 lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size : 47 kDa
    Observed band size : 47 kDa

Anti-NSE antibody [EPR3377] (ab79757)参考文献

This product has been referenced in:
  • Zhang J  et al. AP4M1 is abnormally expressed in oxygen-glucose deprived hippocampal neurons. Neurosci Lett 563:85-9 (2014). IF ; Rat . Read more (PubMed: 24486887) »
  • Fishbein I  et al. Augmentation of phenotype in a transgenic Parkinson mouse heterozygous for a Gaucher mutation. Brain 137:3235-47 (2014). Read more (PubMed: 25351739) »

See all 5 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Cryopreserved embryonic cortical neurons (QBMcells)
Permeabilization No
Specification Cryopreserved embryonic cortical neurons (QBMcells
Fixative paraformaldehyde with picric acid

Ms. Babben Tinner

Verified customer

提交于 Nov 06 2015

Application Western blot
Loading amount 30 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native) (10%)
Sample Rat Tissue lysate - other (hippocampus)
Specification hippocampus
Blocking step Milk as blocking agent for 5 hour(s) and 0 minute(s) · Concentration: 7% · Temperature: 25°C

Mr. House Liu

Verified customer

提交于 Nov 07 2014

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample Zebrafish Tissue sections (retina, neurons)
Specification retina, neurons
Permeabilization Yes - triton X
Fixative Paraformaldehyde

Abcam user community

Verified customer

提交于 Sep 05 2014

I'm sorry about the miss-alignment and thank you for spotting it.

We have a testing discount scheme which you would be eligible to try. This would involve you purchasing ab79757, testing it with your Drosophila samples, and sharing the resul...

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Thank you for contacting us.

The immunogen used to raise anti-NSE antibody (ab79757) is taken from within the region of of human 35 - 60 of enolase2 gamma (NSE) (UniProt: P09104). From my analysis, this shares some homology within the N-term...

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Thank you for your enquiry. I am pleased to provide the following suggested positive controls for these antibodies. I have mainly chosen tissue that has been tested succesfully and shown in an image on the datasheets: 1.Anti-ErbB 2 antibody (ab2428) Hu...

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