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Synthetic peptide derived from human NSE
Our Abpromise guarantee covers the use of ab53025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|ICC/IF||Use a concentration of 1 - 5 µg/ml.|
|WB||1/500 - 1/1000. Predicted molecular weight: 47 kDa.|
|IHC-P||Use at an assay dependent concentration.|
ab53025 staining NSE in Rat brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeablized with 0.3 Triton-X, and blocked with 5% serum for 1 hour at room temperature. The sample was incubated with primary antibody (1/250) at 4°C for 16 hours. Ab96921, DyLight® 594-conjugated Donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
ab53025 staining NSE in mouse brain tissue section by Immunohistochemistry (PFA-perfussion fixed frozen tissue sections). Tissues were collected from mice in each group at 10 weeks. Mice were euthanized, the brains were exposed and removed from the body. Then the brain was cut, on ice and fixed in 4% polyformaldehyde or glutaraldehyde solution for histological examination. The primary antibody was used at 1/75 dilution in PBS and incubated with sample for 2 hours at 37°C. A HRP-conjugated secondary antibody was used at for 1hr at 37°C in a humidity box.