Anti-NSE抗体(ab227301)
Key features and details
- Rabbit polyclonal to NSE
- Suitable for: WB, IHC-Fr, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-NSE抗体
参阅全部 NSE 一抗 -
描述
兔多克隆抗体to NSE -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Fr, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Chicken, Cow, Rhesus monkey -
免疫原
Recombinant fragment within Human NSE (internal sequence). The exact sequence is proprietary.
Database link: P09104 -
阳性对照
- ICC/IF: A431 cells. IHC-Fr: E13.5 rat brain tissue. WB: Mouse brain lysate; HEK-293T and A431 whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab227301于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 47 kDa.
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IHC-Fr |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 47 kDa. |
IHC-Fr
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
靶标
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功能
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival. -
组织特异性
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. -
通路
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5. -
序列相似性
Belongs to the enolase family. -
发展阶段
During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells. -
细胞定位
Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. - Information by UniProt
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数据库链接
- Entrez Gene: 395689 Chicken
- Entrez Gene: 526006 Cow
- Entrez Gene: 2026 Human
- Entrez Gene: 13807 Mouse
- Entrez Gene: 24334 Rat
- Entrez Gene: 714208 Rhesus monkey
- Omim: 131360 Human
- SwissProt: O57391 Chicken
see all -
别名
- 2 phospho D glycerate hydrolyase antibody
- 2-phospho-D-glycerate hydro-lyase antibody
- Eno 2 antibody
see all
图片
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Anti-NSE antibody (ab227301) at 1/10000 dilution + Mouse brain lysate at 50 µg
Predicted band size: 47 kDa10% SDS-PAGE gel.
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A431 (human epidermoid carcinoma cell line) cells stained for NSE (green) using ab227301 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Blue: Hoechst 33342 staining.
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Frozen sectioned E13.5 rat brain tissue stained for NSE using ab227301 at 1/250 dilution in immunohistochemical analysis.
Red: beta Tubulin 3, a mature neuron marker.
Blue: DAPI. -
All lanes : Anti-NSE antibody (ab227301) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 47 kDa10% SDS-PAGE gel.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab227301 尚未被引用在任何文献中。