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Recombinant full length protein purified from E.coli (Human).
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab79757 as a replacement.
Our Abpromise guarantee covers the use of ab16808 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells. (paraformaldehyde fixed cells)
ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|IHC-FoFr||1/100. PubMed: 19279043|
|ICC/IF||Use a concentration of 10 µg/ml.|
|ELISA||Use at an assay dependent concentration.|
|WB||1/2000. Predicted molecular weight: 48 kDa.|
ICC/IF image of ab16808 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab16808 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"