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Our Abpromise guarantee covers the use of ab8324 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [1C1] to NSE (ab8328). For sandwich ELISA, we recommend using this antibody for capture with ab24472 as detection. Can be used as capture antibody in conjunction with ab8328 and ab24472.|
|ICC/IF||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration. Can be paired for ELISA with Mouse monoclonal [1C1] to NSE (ab8328).|
|IHC-FoFr||1/100 - 1/300.|
|IHC-P||Use at an assay dependent concentration. Recommended dilution for immunohistochemistry on formalin-fixed and paraffin-embedded sections is 1:100 - 1:300. Optimal dilution must be determined by experimentation.|
|Flow Cyt||Use 1-2µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 24125751|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"