Anti-NSE抗体[37E4] (ab16807)

概述

  • 产品名称
    Anti-NSE抗体[37E4]
    参阅全部 NSE 一抗
  • 描述
    小鼠单克隆抗体[37E4] to NSE
  • 经测试应用
    适用于: ELISA, WB, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Goat, Chicken, Macaque monkey
  • 免疫原

    Recombinant full length protein purified from E.coli (Human).

  • 阳性对照
    • SH-SY5Y whole cell lysate.
  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

性能

应用

Our Abpromise guarantee covers the use of ab16807 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 48 kDa.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
  • 组织特异性
    The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
  • 通路
    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
  • 序列相似性
    Belongs to the enolase family.
  • 发展阶段
    During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
  • 细胞定位
    Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2 phospho D glycerate hydrolyase antibody
    • 2-phospho-D-glycerate hydro-lyase antibody
    • Eno 2 antibody
    • ENO2 antibody
    • ENOG antibody
    • ENOG_HUMAN antibody
    • Enolase 2 (gamma, neuronal) antibody
    • Enolase 2 antibody
    • Enolase 2 gamma neuronal antibody
    • Enolase2 antibody
    • Epididymis secretory protein Li 279 antibody
    • Gamma enolase antibody
    • Gamma-enolase antibody
    • HEL S 279 antibody
    • Neural enolase antibody
    • Neuron specific enolase antibody
    • Neuron specific gamma enolase antibody
    • Neuron-specific enolase antibody
    • neuronal enriched enolase antibody
    • Neurone specific enolase antibody
    • NSE antibody
    see all

Anti-NSE antibody [37E4] 图像

  • Overlay histogram showing HeLa cells stained with ab16807 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16807, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.


  • Predicted band size : 48 kDa
    ab16807 at a 1/1000 dilution staining approx. 48kDa NSE in 1) SH-SY5Y cell lysate, 2) U87MG cell lysate and 3) mouse brain lysate by Western blot (ECL). ab16807 at a 1/1000 dilution staining approx. 48kDa NSE in 1) SH-SY5Y cell lysate, 2) U87MG cell lysate and 3) mouse brain lysate by Western blot (ECL).

Anti-NSE antibody [37E4] (ab16807)参考文献

ab16807 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. 1) I would expect this antibody to recognize NSE from rat primary brain cultures in WB. 2) The biotinylated antibody would work for an ELISA or IHC, however, for a Western blot I would recommend ab6728 which is an HRP co...

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