Anti-NSE抗体[1C1] (HRP) (ab24472)


  • 产品名称Anti-NSE抗体[1C1] (HRP)
    参阅全部 NSE 一抗
  • 描述
    小鼠单克隆抗体[1C1] to NSE (HRP)
  • 偶联物HRP
  • 特异性Reacts with 45 kD band corresponding to NSE in immunoblotting of human brain extracts
  • 经测试应用适用于: ELISA, Sandwich ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Purified Neuron Specific Enolase from human brain.


Our Abpromise guarantee covers the use of ab24472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution.
Sandwich ELISA Use at an assay dependent dilution. Can be used as detection antibody in conjunction with recommended pair(s).
WB 1/1000.


  • 功能Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
  • 组织特异性The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
  • 通路Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
  • 序列相似性Belongs to the enolase family.
  • 发展阶段During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
  • 细胞定位Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2 phospho D glycerate hydrolyase antibody
    • 2-phospho-D-glycerate hydro-lyase antibody
    • Eno 2 antibody
    • ENO2 antibody
    • ENOG antibody
    • ENOG_HUMAN antibody
    • Enolase 2 (gamma, neuronal) antibody
    • Enolase 2 antibody
    • Enolase 2 gamma neuronal antibody
    • Enolase2 antibody
    • Epididymis secretory protein Li 279 antibody
    • Gamma enolase antibody
    • Gamma-enolase antibody
    • HEL S 279 antibody
    • Neural enolase antibody
    • Neuron specific enolase antibody
    • Neuron specific gamma enolase antibody
    • Neuron-specific enolase antibody
    • neuronal enriched enolase antibody
    • Neurone specific enolase antibody
    • NSE antibody
    see all

Anti-NSE antibody [1C1] (HRP) 图像

  • All lanes : Anti-NSE antibody [1C1] (HRP) (ab24472) at 1/1000 dilution

    Lane 2 : Recombinant Human NSE protein (ab78797) at 5 µg
    Lane 3 : Recombinant Human NSE protein (ab78797) at 0.005 µg
    Lane 4 : Whole tissue lysate prepared from human brain at 15 µg

    Developed using the ECL technique

    Observed band size : 45 kDa (why is the actual band size different from the predicted?)

    Exposure time : 1 minute

    Image courtesy of Dr Rachel Barker by Abreview.

    See Abreview

Anti-NSE antibody [1C1] (HRP) (ab24472)参考文献

ab24472 has not yet been referenced specifically in any publications.

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Thank you for contacting us. I am sorry to hear that you are having trouble using this antibody.

I have read the details provided and suspect that the standard may not be pure enough or may be high conc. of . standard is needed. I have furthe...

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Thank you for your enquiry.

I have investigated our records for ab24472 with my colleagues and the originator. I can confirm that regrettably there is an error on the datasheet, and this antibody does not contain sodium azide.


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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (brain)
Loading amount 15 µg
Specification brain
Gel Running Conditions Reduced Denaturing (4-20% Tris-HCl)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Dr. Rachel Barker

Verified customer

提交于 Mar 21 2012