概述

  • 产品名称
  • 描述
    兔多克隆抗体to NSD3
  • 特异性
    This antibody should recognise both the 72 and 161 kDa isoforms: NP_060248 (645 aa) - 72.6 kDa, NP_075447 (1437 aa) - 161 kDa.
  • 经测试应用
    适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide derived from residues 500 - 600 of Human NSD3.

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HeLa, HEK293.

      This antibody gave a positive signal in the following tissues: Formalin Fixed Paraffin Embedded Human Breast Carcinoma.

      This antibody gave a positive signal in the following cell lines: HeLa.

性能

应用

Our Abpromise guarantee covers the use of ab4514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 72.6, 161 kDa (predicted molecular weight: 72.6 , 161 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

靶标

  • 功能
    Histone methyltransferase. Preferentially methylates 'Lys-4' and 'Lys-27' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation, while 'Lys-27' is a mark for transcriptional repression.
  • 组织特异性
    Highly expressed in brain, heart and skeletal muscle. Expressed at lower level in liver and lung.
  • 疾病相关
    Defects in WHSC1L1 may be involved in non small cell lung carcinomas (NSCLC). Amplified or overexpressed in NSCLC.
    A chromosomal aberration involving WHSC1L1 is found in childhood acute myeloid leukemia. Translocation t(8;11)(p11.2;p15) with NUP98.
  • 序列相似性
    Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. SET2 subfamily.
    Contains 1 AWS domain.
    Contains 4 PHD-type zinc fingers.
    Contains 1 post-SET domain.
    Contains 2 PWWP domains.
    Contains 1 SET domain.
  • 细胞定位
    Nucleus. Chromosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp667H044 antibody
    • FLJ20353 antibody
    • Histone lysine N methyltransferase NSD3 antibody
    • Histone-lysine N-methyltransferase NSD3 antibody
    • MGC126766 antibody
    • MGC142029 antibody
    • NSD 3 antibody
    • NSD3_HUMAN antibody
    • Nuclear set domain containing 3 antibody
    • Nuclear SET domain containing protein 3 antibody
    • Nuclear SET domain-containing protein 3 antibody
    • pp14328 antibody
    • Protein whistle antibody
    • Whistle antibody
    • WHSC1 like protein 1 antibody
    • WHSC1-like 1 isoform 9 with methyltransferase activity to lysine antibody
    • WHSC1-like protein 1 antibody
    • WHSC1L1 antibody
    • WHSC1L1 protein antibody
    • Wolf Hirschhorn syndrome candidate 1 like 1 antibody
    • Wolf-Hirschhorn syndrome candidate 1-like protein 1 antibody
    see all

图片

  • Breast carcinoma tissue was fixed in 10% formalin at 4°C overnight and paraffin embedded. Following blocking the tissue sections were incubated with ab4514 (1/50 dilution) at 37°C for 1 hour, and with the secondary antibody at 37°C for 30 minutes. The slides were counterstained with Hematoxylin. Washes were carried out using TBS. Heat mediated antigen retrieval was carried out using the Microwave method before commencing the staining protocol. ab4514 staining appears to be localized mainly to the cytoplasm. Although the putative cellular localization of NSD3 is the nucleus we have found no evidence in the current literature to confirm this and we believe ab4514 may be detecting a cytoplasmic form of the protein. We would be very grateful for any customer feedback about NSD3 and ab4514.


  • All lanes : Anti-NSD3 antibody (ab4514) at 1 µg/ml

    Lane 1 : HeLa whole cell extract
    Lane 2 : HEK293 cell extract
    Lane 3 : HeLa whole cell extract with Human NSD3 peptide (ab14995) at 1 µg/ml
    Lane 4 : HEK293 cell extract with Human NSD3 peptide (ab14995) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.


    Predicted band size : 72.6 , 161 kDa
    Observed band size : 72.6,161 kDa
    Additional bands at : 115 kDa (possible cross reactivity).
  • ICC/IF image of ab4514 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab4514, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

    As seen in Immunohistochemical analysis (shown above), although the putative cellular localization of NSD3 is the nucleus we have found no evidence in the current literature to confirm this and we believe ab4514 may be detecting a cytoplasmic form of the protein.

文献

This product has been referenced in:
  • Rahman S  et al. The Brd4 extraterminal domain confers transcription activation independent of pTEFb by recruiting multiple proteins, including NSD3. Mol Cell Biol 31:2641-52 (2011). Read more (PubMed: 21555454) »

See 1 Publication for this product

客户评价及客户问答

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Abreviews
Application
Immunoprecipitation
Immuno-precipitation step
Protein A
Sample
Human Cell lysate - nuclear (Hela)
Specification
Hela
Total protein in input
4e+007 cells
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提交于 Jun 03 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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