Anti-NSD3抗体[2E9] (ab66745)

概述

  • 产品名称Anti-NSD3抗体[2E9]
    参阅全部 NSD3 一抗
  • 描述
    小鼠单克隆抗体[2E9] to NSD3
  • 经测试应用适用于: WB, ELISA, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment corresponding to amino acids 383-660 of Human NSD3 purified from E. coli

  • 阳性对照
    • HeLa cell lysate
  • 常规说明


    ab66745, clone 2E9, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human NSD3 protein.

性能

应用

Our Abpromise guarantee covers the use of ab66745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/3000. Detects a band of approximately 72 kDa. (predicted molecular weight for the full length protein: 162 kDa).
ELISA Use at an assay dependent concentration.
Flow Cyt Use 0.1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Histone methyltransferase. Preferentially methylates 'Lys-4' and 'Lys-27' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation, while 'Lys-27' is a mark for transcriptional repression.
  • 组织特异性Highly expressed in brain, heart and skeletal muscle. Expressed at lower level in liver and lung.
  • 疾病相关Defects in WHSC1L1 may be involved in non small cell lung carcinomas (NSCLC). Amplified or overexpressed in NSCLC.
    A chromosomal aberration involving WHSC1L1 is found in childhood acute myeloid leukemia. Translocation t(8;11)(p11.2;p15) with NUP98.
  • 序列相似性Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. SET2 subfamily.
    Contains 1 AWS domain.
    Contains 4 PHD-type zinc fingers.
    Contains 1 post-SET domain.
    Contains 2 PWWP domains.
    Contains 1 SET domain.
  • 细胞定位Nucleus. Chromosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp667H044 antibody
    • FLJ20353 antibody
    • Histone lysine N methyltransferase NSD3 antibody
    • Histone-lysine N-methyltransferase NSD3 antibody
    • MGC126766 antibody
    • MGC142029 antibody
    • NSD 3 antibody
    • NSD3_HUMAN antibody
    • Nuclear set domain containing 3 antibody
    • Nuclear SET domain containing protein 3 antibody
    • Nuclear SET domain-containing protein 3 antibody
    • pp14328 antibody
    • Protein whistle antibody
    • Whistle antibody
    • WHSC1 like protein 1 antibody
    • WHSC1-like 1 isoform 9 with methyltransferase activity to lysine antibody
    • WHSC1-like protein 1 antibody
    • WHSC1L1 antibody
    • WHSC1L1 protein antibody
    • Wolf Hirschhorn syndrome candidate 1 like 1 antibody
    • Wolf-Hirschhorn syndrome candidate 1-like protein 1 antibody
    see all

Anti-NSD3 antibody [2E9] 图像

  • Overlay histogram showing HeLa cells stained with ab66745 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab66745, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Anti-NSD3 antibody [2E9] (ab66745) at 1/2000 dilution + HeLa cell lysate at 40 µg

    Secondary
    HRP-conjugated goat anti-mouse
    Developed using the ECL technique

    Observed band size : 72 kDa (why is the actual band size different from the predicted?)

Anti-NSD3 antibody [2E9] (ab66745)参考文献

ab66745 has not yet been referenced specifically in any publications.

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I have been in touch with the supplier regading thisproduct. Unfortunatley they have not tested to determine wheter this reacts with each isoform of NSD3 and cannot provide any further immunogen information. I ...

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