概述

  • 产品名称NS 398
  • 描述
    COX-2 inhibitor
  • 生物学描述Selective cyclooxygenase-2 inhibitor (IC50 values are 3.8 and >100 μM for COX-2 and COX-1, respectively). Orally active. Anti-inflammatory, antipyretic, analgesic and non-ulcerogenic in vivo. Induces apoptosis and cell cycle arrest.
  • 纯度> 98%

性能

    应用

    Our Abpromise guarantee covers the use of ab120295 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Functional Studies Use at an assay dependent concentration.

    NS 398 图像

    • ab32034 staining p27 KIP1 in MCF7 cells treated with NS 398 (ab120295), by ICC/IF. Increase in p27 KIP1 expression correlates with increased concentration of NS 398, as described in literature.
      The cells were incubated at 37°C for 6h in media containing different concentrations of ab120295 (NS 398) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab32034 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.

    NS 398 (ab120295)参考文献

    This product has been referenced in:
    • Du Y  et al. Induction of apoptosis and cell cycle arrest by NS398 in oral squamous cell carcinoma cells via downregulation of E2 promoter-binding factor-1. Oncol Rep 20:605-11 (2008). Read more (PubMed: 18695912) »
    • Futaki N  et al. NS-398, a new anti-inflammatory agent, selectively inhibits prostaglandin G/H synthase/cyclooxygenase (COX-2) activity in vitro. Prostaglandins 47:55-9 (1994). Read more (PubMed: 8140262) »
    • Futaki N  et al. NS-398, a novel non-steroidal anti-inflammatory drug with potent analgesic and antipyretic effects, which causes minimal stomach lesions. Gen Pharmacol 24:105-10 (1993). Read more (PubMed: 8482483) »

    See 0 Publications for this product

    Product Wall

    Thank you for your reply.

    That is the problem with this target. I have been able to find chemical inhibitors of VEGFR but not for VEGF. There seems to be mainly antibody inhibitors of VEGF. If you do find a compound you are interested in, ple...

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    I hope your grant application is going well. We have several antibodies against the targets you have mentioned which may be of interest to you:

    HO-1

    I would suggest looking at the rabbit polyclonal http://www.abcam.com/Heme-Oxygenas...

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    Thank you for your reply.

    I am happy to confirm that we have a COX2 inhibitor in our catalog.

    ab120295 (NS 398)

    http://www.abcam.com/index.html?datasheet=120295 (or use the following: http://www.abcam.com/index.html?datash...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"